Analysis of cell surface and relative gene expression of heat shock protein 70 in human leukemia cell lines
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
18297536
DOI
10.1080/10428190701851372
PII: 790795244
Knihovny.cz E-zdroje
- MeSH
- antigeny povrchové analýza MeSH
- buňky K562 MeSH
- Jurkat buňky MeSH
- leukemie patologie MeSH
- lidé MeSH
- membránové proteiny analýza MeSH
- messenger RNA analýza MeSH
- nádorové buněčné linie MeSH
- polymerázová řetězová reakce MeSH
- proteiny tepelného šoku HSP70 analýza genetika MeSH
- průtoková cytometrie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antigeny povrchové MeSH
- membránové proteiny MeSH
- messenger RNA MeSH
- proteiny tepelného šoku HSP70 MeSH
Hsp70 was found to be expressed on the cell surface of leukemia cell lines compared with normal cells. Hsp70 cell surface and mRNA expression was studied in K562, Jurkat and CCRF-CEM human leukemia cell lines during several passages of in vitro culture. All the cell lines were positive for cell surface Hsp70 expression. However, increased mRNA expression was observed only in K562 cells where the expression was 9.88-fold higher on average compared with healthy controls. Jurkat cells, however, showed a significant negative correlation between cell surface and mRNA Hsp70 expression; high cell surface expression correlated with low mRNA expression in the Jurkat cell line.
Citace poskytuje Crossref.org
Heat-shock protein expression in leukemia
