Composition and electrophysiological activity of constituents identified in male wing gland secretion of the bumblebee parasite Aphomia sociella
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
19072711
DOI
10.1021/np800434x
PII: 10.1021/np800434x
Knihovny.cz E-resources
- MeSH
- Behavior, Animal physiology MeSH
- Phenylethyl Alcohol chemistry isolation & purification MeSH
- Hexanols chemistry isolation & purification MeSH
- Isocoumarins chemistry isolation & purification MeSH
- Wings, Animal metabolism MeSH
- Fatty Acids chemistry isolation & purification MeSH
- Molecular Structure MeSH
- Moths * chemistry physiology MeSH
- Ochratoxins chemistry isolation & purification MeSH
- Gas Chromatography-Mass Spectrometry MeSH
- Sex Attractants physiology MeSH
- Stereoisomerism MeSH
- Bees parasitology MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 1-hexanol MeSH Browser
- Phenylethyl Alcohol MeSH
- Hexanols MeSH
- Isocoumarins MeSH
- Fatty Acids MeSH
- ochracin MeSH Browser
- Ochratoxins MeSH
- Sex Attractants MeSH
Male wing gland secretion and volatiles emanating from calling males were investigated in the bumble bee wax moth, Aphomia. sociella, using comprehensive two-dimensional gas chromatography-mass spectrometry (GCxGC-TOF-MS), gas chromatography-infrared spectroscopy (GC-FTIR), enantioselective gas chromatography, electroantennography (EAG), gas chromatography with electroantennographic detection (GC-EAD), and NMR. GC-EAD analysis of the male wing gland secretion revealed seven active areas, corresponding to 1-hexanol (1), 2-phenylethanol (2), [(R),(Z)]-nona-2,6-dien-4-olide (3), [(S),(Z)]-nona-6-en-4-olide (4), mellein (5), phytone (6), and a mixture of C(18) fatty acids (7). Solid-phase microextraction (SPME) confirmed the presence of 2-phenylethanol, nona-2,6-dien-4-olide, nona-6-en-4-olide, mellein, and phytone in volatiles emanating from a calling male. Though the abundance of these compounds slightly differed in SPME and gland secretion analysis, nona-2,6-dien-4-olide and mellein dominated in both samples, followed by 2-phenylethanol, nona-6-en-4-olide, and phytone. The strong antennal responses elicited by components of the secretion suggest that one or more of these compounds constitute the sex pheromone. Both sexes perceived male wing gland secretion, with females being significantly more sensitive compared to males.
References provided by Crossref.org
