Concatenated SSU and LSU rDNA data confirm the main evolutionary trends within myxosporeans (Myxozoa: Myxosporea) and provide an effective tool for their molecular phylogenetics
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
19477283
DOI
10.1016/j.ympev.2009.05.018
PII: S1055-7903(09)00202-4
Knihovny.cz E-resources
- MeSH
- Bayes Theorem MeSH
- DNA Primers MeSH
- Phylogeny * MeSH
- Genes, rRNA MeSH
- Evolution, Molecular * MeSH
- Myxozoa classification genetics MeSH
- Likelihood Functions MeSH
- DNA, Ribosomal genetics MeSH
- Sequence Analysis, DNA MeSH
- Sequence Alignment MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA Primers MeSH
- DNA, Ribosomal MeSH
Views on myxosporean phylogeny and systematics have recently undergone substantial changes resulting from analyses of SSU rDNA. Here, we further investigate the evolutionary trends within myxosporean lineages by using 35 new sequences of the LSU rDNA. We show a good agreement between the two rRNA genes and confirm the main phylogenetic split between the freshwater and marine lineages. The informative superiority of the LSU data is shown by an increase of the resolution, nodal supports and tree indexes in the LSU rDNA and combined analyses. We determine the most suitable part of LSU for the myxosporean phylogeny by comparing informative content in various regions of the LSU sequences. Based on this comparison, we propose the D5-3'-end part of the LSU rRNA gene as the most informative region which provides in concatenation with the complete SSU a well resolved and robust tree. To allow for simple amplification of the marker, we design specific primer set for this part of LSU rDNA.
References provided by Crossref.org
Myxozoa in high Arctic: Survey on the central part of Svalbard archipelago
History of myxozoan character evolution on the basis of rDNA and EF-2 data
