New haemolytic potential assay on an alternative insect model
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
19627281
DOI
10.1111/j.1742-7843.2009.00446.x
PII: PTO446
Knihovny.cz E-resources
- MeSH
- Animal Testing Alternatives MeSH
- Phenylhydrazines toxicity MeSH
- Hemocytes cytology drug effects MeSH
- Hemolysis * MeSH
- Larva cytology MeSH
- Models, Animal * MeSH
- Cell Count MeSH
- Spodoptera cytology MeSH
- Toxicity Tests methods MeSH
- Xenobiotics toxicity MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Phenylhydrazines MeSH
- phenylhydrazine MeSH Browser
- Xenobiotics MeSH
A new screening test of a haemolytic potential is presented on Spodoptera larvae in vivo. The assay was evaluated using phenylhydrazine, the well-known xenobiotic which induces haemolysis in mammals. Haemocyte disintegration causes changes in total haemocyte counts and differentials. It is shown that only qualitative evaluation of panoptically stained haemolymph smears is sufficient, fast and cheap to detect haemolytic potential. The presented assay also prevents unneeded, non-ethical use of vertebrates.
References provided by Crossref.org
