A TNF-like trimeric lectin domain from Burkholderia cenocepacia with specificity for fucosylated human histo-blood group antigens
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
20152153
DOI
10.1016/j.str.2009.10.021
PII: S0969-2126(09)00470-5
Knihovny.cz E-resources
- MeSH
- Blood Group Antigens chemistry immunology metabolism MeSH
- Bacterial Proteins chemistry metabolism MeSH
- Burkholderia chemistry metabolism MeSH
- Epitopes chemistry immunology metabolism MeSH
- Fucose chemistry metabolism MeSH
- Protein Structure, Quaternary MeSH
- Lectins chemistry metabolism MeSH
- Humans MeSH
- Models, Molecular MeSH
- Molecular Sequence Data MeSH
- Amino Acid Sequence MeSH
- Sequence Alignment MeSH
- Binding Sites MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Blood Group Antigens MeSH
- Bacterial Proteins MeSH
- Epitopes MeSH
- Fucose MeSH
- Lectins MeSH
The opportunistic pathogen Burkholderia cenocepacia expresses several soluble lectins, among them BC2L-C. This lectin exhibits two domains: a C-terminal domain with high sequence similarity to the recently described calcium-dependent mannose-binding lectin BC2L-A, and an N-terminal domain of 156 amino acids without similarity to any known protein. The recombinant N-terminal BC2L-C domain is a new lectin with specificity for fucosylated human histo-blood group epitopes H-type 1, Lewis b, and Lewis Y, as determined by glycan array and isothermal titration calorimetry. Methylselenofucoside was used as ligand to solve the crystal structure of the N-terminal BC2L-C domain. Additional molecular modeling studies rationalized the preference for Lewis epitopes. The structure reveals a trimeric jellyroll arrangement with striking similarity to TNF-like proteins, and to BclA, the spore protein from Bacillus anthracis which may play an important role in bioadhesion of anthrax spores in human lungs.
References provided by Crossref.org
Glycomimetics for the inhibition and modulation of lectins
Development of 48-condition buffer screen for protein stability assessment
