Anxiety-like behavior in the elevated-plus maze tests and enhanced IL-1β, IL-6, NADPH oxidase-1, and iNOS mRNAs in the hippocampus during early stage of adjuvant arthritis in rats
Language English Country Ireland Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
20970480
DOI
10.1016/j.neulet.2010.10.032
PII: S0304-3940(10)01371-6
Knihovny.cz E-resources
- MeSH
- Arthritis, Experimental complications metabolism psychology MeSH
- Maze Learning physiology MeSH
- Hippocampus metabolism MeSH
- Interleukin-1beta biosynthesis MeSH
- Interleukin-6 biosynthesis MeSH
- Rats MeSH
- RNA, Messenger biosynthesis MeSH
- NADH, NADPH Oxidoreductases biosynthesis MeSH
- NADPH Oxidase 1 MeSH
- Rats, Inbred Lew MeSH
- Nitric Oxide Synthase Type II biosynthesis MeSH
- Anxiety complications metabolism psychology MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Interleukin-1beta MeSH
- Interleukin-6 MeSH
- RNA, Messenger MeSH
- NADH, NADPH Oxidoreductases MeSH
- NADPH Oxidase 1 MeSH
- Nos2 protein, rat MeSH Browser
- NOX1 protein, rat MeSH Browser
- Nitric Oxide Synthase Type II MeSH
We studied anxiety-like behavior in the elevated plus-maze (EPM) tests in male Lewis rats on days 2 and 4 of adjuvant arthritis (AA). In plasma we analyzed C-reactive protein (CRP), albumin, ACTH, corticosterone, in the hippocampus the mRNA expression of interleukin-1β (IL-1β), interleukin-6 (IL-6), corticotrophin releasing factor (CRH), NADPH oxidases NOX1 and NOX2, and inducible NO-synthase (iNOS). EPM tests showed a higher anxiety index in AA rats on days 2 and 4 and reduction of total entries. On days 2 and 4 we found reduced plasma albumin, enhanced CRP, ACTH and corticosterone, and in the hippocampus enhanced mRNA for NOX1 and IL-1β in AA rats, on day 4 we found enhanced mRNAs for iNOS and IL-6, and reduced mRNA for CRH. The mRNA for NOX2 did not change on any experimental day. These results suggest enhanced anxiety, as well as locomotor impairment during the early phase of AA that correlate with enhanced mRNA expressions of parameters of oxidative stress NOX1, iNOS, and inflammatory cytokines IL-1β and IL-6 in the hippocampus.
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