Reliability of nucleotide sequence information of full-length flagellin A gene (flaA) and flaA short variable region (SVR) for molecular discrimination of Campylobacter lari organisms
Language English Country United States Media print-electronic
Document type Journal Article
- MeSH
- Campylobacter jejuni classification genetics isolation & purification MeSH
- Campylobacter lari classification genetics isolation & purification MeSH
- DNA, Bacterial chemistry genetics MeSH
- Flagellin genetics MeSH
- Genotype MeSH
- Humans MeSH
- Molecular Sequence Data MeSH
- Polymorphism, Genetic * MeSH
- Sequence Analysis, DNA MeSH
- Cluster Analysis MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- DNA, Bacterial MeSH
- flaA protein, bacteria MeSH Browser
- Flagellin MeSH
We aimed to clarify if the thermophilic Campylobacter lari organisms including urease-negative (UN) C. lari and urease-positive thermophilic Campylobacter (UPTC) can be differentiated at the species and/or subspecies levels by employing the full-length flaA gene and flaA short variable region (SVR) nucleotide sequence information or not. Thermophilic Campylobacter isolates (n = 45) including UN C. lari (n = 17), UPTC (n = 18), and Campylobacter jejuni (n = 10) were well discriminated at the isolate level by the unweighted pair group method using arithmetic means analysis and neighbor joining procedures constructed based on the full-length flaA gene and flaA SVR nucleotide sequence information. Thus, these procedures may possibly be useful for epidemimological studies for C. lari and C. jejuni.
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