Molecular evidence of Plesiomonas shigelloides as a possible zoonotic agent
Language English Country United States Media print-electronic
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Genetic Variation MeSH
- Gram-Negative Bacterial Infections microbiology transmission veterinary MeSH
- DNA, Intergenic MeSH
- Humans MeSH
- Environmental Microbiology MeSH
- Molecular Typing methods MeSH
- Inverted Repeat Sequences MeSH
- Plesiomonas classification genetics isolation & purification MeSH
- Electrophoresis, Gel, Pulsed-Field methods MeSH
- Repetitive Sequences, Nucleic Acid MeSH
- Random Amplified Polymorphic DNA Technique methods MeSH
- Zoonoses microbiology transmission MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Intergenic MeSH
The most frequently used method for establishing epidemiological relationships between Plesiomonas shigelloides strains is O:H serotyping. However, a number of strains are not serotypeable and isolates from diverse sources can display the same serovar. Moreover, since the zoonotic nature of Plesiomonas has been suggested and this hypothesis is based on the identical serovars found in animals and humans, we intend to use four DNA-based techniques: random amplified polymorphic DNA-PCR, enterobacterial repetitive intergenic consensus-PCR, repetitive extragenic palindromic-PCR, and pulsed field gel electrophoresis in order to screen 24 strains belonging to nine O:H serovars isolated from humans, animals, and the environment. In general, P. shigelloides showed a high genetic heterogeneity. Three pairs of strains, each containing a human and an animal isolate, displayed similar genotypes. This is the first report that provides molecular evidence that P. shigelloides may be zoonotic.
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