Influence of collagen and chondroitin sulfate (CS) coatings on poly-(lactide-co-glycolide) (PLGA) on MG 63 osteoblast-like cells
Language English Country Czech Republic Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
21812519
DOI
10.33549/physiolres.931994
PII: 931994
Knihovny.cz E-resources
- MeSH
- Coated Materials, Biocompatible chemistry pharmacology MeSH
- Cell Engineering methods MeSH
- Cell Line MeSH
- Chondroitin Sulfates chemistry pharmacology MeSH
- Collagen Type I chemistry pharmacology MeSH
- Polylactic Acid-Polyglycolic Acid Copolymer MeSH
- Cells, Cultured MeSH
- Lactic Acid chemistry MeSH
- Polyglycolic Acid chemistry MeSH
- Humans MeSH
- Osteoblasts cytology drug effects physiology MeSH
- Osteogenesis drug effects physiology MeSH
- Cell Proliferation drug effects MeSH
- Materials Testing MeSH
- Tissue Scaffolds MeSH
- Cell Survival drug effects MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Coated Materials, Biocompatible MeSH
- Chondroitin Sulfates MeSH
- Collagen Type I MeSH
- Polylactic Acid-Polyglycolic Acid Copolymer MeSH
- Lactic Acid MeSH
- Polyglycolic Acid MeSH
Poly-(lactide-co-glycolide) (PLGA) is an FDA-approved biodegradable polymer which has been widely used as a scaffold for tissue engineering applications. Collagen has been used as a coating material for bone contact materials, but relatively little interest has focused on biomimetic coating of PLGA with extracellular matrix components such as collagen and the glycosaminoglycan chondroitin sulfate (CS). In this study, PLGA films were coated with collagen type I or collagen I with CS (collagen I/CS) to investigate the effect of CS on the behaviour of the osteoblastic cell line MG 63. Collagen I/CS coatings promoted a significant increase in cell number after 3 days (in comparison to PLGA) and after 7 days (in comparison to PLGA and collagen-coated PLGA). No influence of collagen I or collagen I/CS coatings on the spreading area after 1 day of culture was observed. However, the cells on collagen I/CS formed numerous filopodia and displayed well developed vinculin-containing focal adhesion plaques. Moreover, these cells contained a significantly higher concentration of osteocalcin, measured per mg of protein, than the cells on the pure collagen coating. Thus, it can be concluded that collagen I/CS coatings promote MG 63 cell proliferation, improve cell adhesion and enhance osteogenic cell differentiation.
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