Insights into the pH up-shift responsive mechanism of Acidithiobacillus ferrooxidans by microarray transcriptome profiling
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Acidithiobacillus genetics metabolism MeSH
- Genes, Bacterial * MeSH
- Nitrogen Fixation genetics MeSH
- Phosphates metabolism MeSH
- Stress, Physiological MeSH
- Genome, Bacterial * MeSH
- Hydrogen-Ion Concentration MeSH
- Carbon Dioxide metabolism MeSH
- Proteomics methods MeSH
- Industrial Microbiology methods MeSH
- Gene Expression Regulation, Bacterial physiology MeSH
- Oligonucleotide Array Sequence Analysis MeSH
- Sulfur metabolism MeSH
- Gene Expression Profiling MeSH
- Hydrogen metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Phosphates MeSH
- Carbon Dioxide MeSH
- Sulfur MeSH
- Hydrogen MeSH
To define the molecular response of Acidithiobacillus ferrooxidans under pH up-shift, temporal gene expression profiles were examined by using whole-genome DNA microarrays for A. ferrooxidans. Approximately 30% of the 3,132 genes represented on the microarray were significantly upregulated over a 160-min period, while about 14% were significantly downregulated. Our results revealed that A. ferrooxidans showed potential self-protection and self-regulation performance in response to pH up-shift stress. Many genes involved in regulation of membrane components were differentially expressed under the pH up-shift stress. Likewise, most of genes involved in phosphate metabolism, sulfur assimilation, and CO(2) fixation were obviously induced. Conversely, the transcription of a polyphosphate kinase gene (AFE1210) associated with phosphate storage was significantly repressed, which probably stemmed from the depletion of polyphosphate. Besides, most of the genes involved in hydrogen uptake were significantly induced, whereas many genes involved in nitrogen fixation were obviously repressed, which suggested that hydrogen uptake and nitrogen fixation could contribute to cytoplasmic pH homeostasis.
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Nucleic Acids Res. 2000 Nov 1;28(21):4317-31 PubMed
Arch Microbiol. 2005 Sep;183(6):411-20 PubMed
Biochim Biophys Acta. 2001 May 1;1505(1):144-57 PubMed
Trends Microbiol. 2007 Apr;15(4):165-71 PubMed
J Bacteriol. 1999 Aug;181(16):5085-9 PubMed
J Bacteriol. 2002 Jul;184(14):4025-32 PubMed
Microb Cell Fact. 2005 May 06;4(1):13 PubMed
Bioinformatics. 2002 Nov;18(11):1432-7 PubMed
Adv Microb Physiol. 2009;55:1-79, 317 PubMed
Proc Natl Acad Sci U S A. 2001 Aug 28;98(18):10416-21 PubMed
J Bacteriol. 1999 Apr;181(8):2394-402 PubMed
Appl Environ Microbiol. 1999 Jul;65(7):2857-62 PubMed
Biochim Biophys Acta. 2005 Nov 30;1717(2):67-88 PubMed
Biochem Biophys Res Commun. 1995 Feb 27;207(3):978-84 PubMed
BMC Genomics. 2008 Dec 11;9:597 PubMed
Mol Microbiol. 1994 Dec;14(5):939-46 PubMed
J Bacteriol. 2005 Jan;187(1):304-19 PubMed
Antonie Van Leeuwenhoek. 1984;50(5-6):473-87 PubMed
Appl Environ Microbiol. 2008 Mar;74(6):1829-35 PubMed
J Bacteriol. 1996 May;178(10):2853-60 PubMed
Appl Environ Microbiol. 1990 Sep;56(9):2922-3 PubMed
Extremophiles. 1998 May;2(2):67-74 PubMed
Folia Biol (Praha). 2009;55(1):1-6 PubMed
Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):12170-5 PubMed
J Bacteriol. 1991 Jan;173(2):910-5 PubMed
Appl Microbiol Biotechnol. 2003 Dec;63(3):249-57 PubMed
J Bacteriol. 2003 Mar;185(6):2009-16 PubMed
BMC Genomics. 2003 Dec 15;4(1):51 PubMed
Nat Rev Genet. 2006 Jan;7(1):55-65 PubMed
Biochemistry. 2007 Apr 3;46(13):4077-89 PubMed
Mol Microbiol. 2001 Jul;41(1):59-71 PubMed
J Bacteriol. 1987 Feb;169(2):885-7 PubMed
J Microbiol Biotechnol. 2008 Nov;18(11):1747-54 PubMed
J Bacteriol. 2004 Nov;186(22):7796-803 PubMed
Plant Cell. 2001 Apr;13(4):793-806 PubMed
J Bacteriol. 2006 Jun;188(12):4560-9 PubMed
Appl Microbiol Biotechnol. 2003 Dec;63(3):239-48 PubMed
Appl Environ Microbiol. 2008 Sep;74(17):5276-84 PubMed
J Biol Chem. 1994 Nov 4;269(44):27365-71 PubMed
J Bacteriol. 1997 Jun;179(12):3851-7 PubMed
J Bacteriol. 2003 Aug;185(16):5019-22 PubMed
Appl Environ Microbiol. 2004 Aug;70(8):4613-20 PubMed
Proc Natl Acad Sci U S A. 1998 Oct 13;95(21):12474-9 PubMed
J Biol Chem. 1988 Feb 15;263(5):2409-16 PubMed
Arch Microbiol. 1975 Oct 27;105(2):123-7 PubMed
Biotechnol Adv. 2006 Mar-Apr;24(2):197-211 PubMed
J Bacteriol. 2005 Apr;187(7):2501-7 PubMed
Mol Microbiol. 1999 Dec;34(5):995-1006 PubMed
J Biol Chem. 2006 Dec 1;281(48):36632-42 PubMed
Biochim Biophys Acta. 1981 Dec;650(2-3):151-66 PubMed
