Isolation and cholinesterase activity of Amaryllidaceae alkaloids from Nerine bowdenii
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
22312717
Knihovny.cz E-resources
- MeSH
- Alkaloids chemistry isolation & purification pharmacology MeSH
- Cholinesterase Inhibitors chemistry isolation & purification pharmacology MeSH
- Humans MeSH
- Liliaceae chemistry MeSH
- Gas Chromatography-Mass Spectrometry MeSH
- Plant Extracts analysis MeSH
- Dose-Response Relationship, Drug MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Alkaloids MeSH
- Cholinesterase Inhibitors MeSH
- Plant Extracts MeSH
Amaryllidaceae species are known as ornamental plants. Some contain galanthamine, an acetylcholinesterase inhibitor. The chemical composition of the alkaloid extract of bulbs of Nerine bowdenii Watson has been analyzed by means of GC/MS. Twenty-two compounds were detected and nineteen of them identified, one of which was belladine. The alkaloid extract showed promising cholinesterase inhibitory activities against human blood acetylcholinesterase (HuAChE; IC50 = 87.9 +/- 3.5 microg/mL) and human plasma butyrylcholinesterase (HuBuChE; IC50 = 14.8 +/- 1.1 microg/mL). Belladine inhibited HuAChE and HuBuChE in a dose-dependent manner with IC50 values of 781 +/- 12.5 microM and 284.8 +/-4.2 microM, respectively.
The Genus Nerine Herb. (Amaryllidaceae): Ethnobotany, Phytochemistry, and Biological Activity
