An enzymatic glycosylation of nucleoside analogues using β-galactosidase from Escherichia coli
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
22465858
DOI
10.1016/j.bmc.2012.02.062
PII: S0968-0896(12)00205-2
Knihovny.cz E-resources
- MeSH
- beta-Galactosidase metabolism MeSH
- Biocatalysis MeSH
- Escherichia coli enzymology MeSH
- Glycosylation MeSH
- Kinetics MeSH
- Lactose metabolism MeSH
- Nucleosides chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- beta-Galactosidase MeSH
- Lactose MeSH
- Nucleosides MeSH
A new enzymatic method for the synthesis of β-galactosides of nucleosides and acyclic nucleoside analogues has been developed, using β-galactosidase from Escherichia coli as a catalyst and lactose as a sugar donor. The method is very rapid, feasible and last but not least inexpensive. Its applicability has been proven for a broad variety of possible substrates with respect to its scaling up for preparative use. Five new compounds from a series of nucleoside and acyclic nucleoside analogues have been prepared on a scale of several hundred milligrams, in all cases revealing very good results of the method concerning the reproducibility of the reaction yields and simplicity of the purification process.
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