Rhodococcus sp. Q5, a novel agarolytic bacterium isolated from printing and dyeing wastewater
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Agar metabolism MeSH
- Bacterial Proteins genetics metabolism MeSH
- Sodium Chloride metabolism MeSH
- Phylogeny MeSH
- Glycoside Hydrolases genetics metabolism MeSH
- Molecular Sequence Data MeSH
- Wastewater microbiology MeSH
- Industrial Waste analysis MeSH
- Rhodococcus classification genetics isolation & purification metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Agar MeSH
- agarase MeSH Browser
- Bacterial Proteins MeSH
- Sodium Chloride MeSH
- Glycoside Hydrolases MeSH
- Waste Water MeSH
- Industrial Waste MeSH
An agar-degrading bacterium, Rhodococcus sp. Q5, was isolated from printing and dyeing wastewater using a mineral salts agar plate containing agar as the sole carbon source. The bacterium grew from pH 4.0 to 9.0, from 15 to 35°C, and in NaCl concentrations of 0-5 %; optimal values were pH 6.0, 30°C, and 1 % NaCl. Maximal agarase production was observed at pH 6.0 and 30°C. The bacterium did not require NaCl for growth or agarase production. The agarase secreted by Q5 was inducible by agar and was repressed by all simple sugars tested except lactose. Strain Q5 could hydrolyze starch but not cellulose or carboxymethyl cellulose. Agarase activity could also be detected in the medium when lactose or starch was the sole source of carbon and energy. Strain Q5 could grow in nitrogen-free mineral media; an organic nitrogen source was more effective than inorganic carbon sources for growth and agarase production. Addition of more organic nitrogen (peptone) to the medium corresponded with reduced agarase activity.
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Appl Environ Microbiol. 1998 Nov;64(11):4378-83 PubMed
Eur J Biochem. 1983 Oct 3;135(3):553-8 PubMed
Anal Biochem. 1976 May 7;72:248-54 PubMed
Antonie Van Leeuwenhoek. 1978;44(1):105-13 PubMed
Appl Microbiol Biotechnol. 2006 Jun;71(1):23-33 PubMed
J Appl Microbiol. 2009 Jan;106(1):181-90 PubMed
J Bacteriol. 1942 Nov;44(5):555-70 PubMed
J Gen Microbiol. 1975 Mar;87(1):150-8 PubMed
Antonie Van Leeuwenhoek. 1975;41(4):431-47 PubMed
Antonie Van Leeuwenhoek. 1974;40(3):329-46 PubMed
J Microbiol Biotechnol. 2010 Oct;20(10):1378-85 PubMed
Appl Microbiol Biotechnol. 2008 Feb;78(2):265-73 PubMed
Appl Environ Microbiol. 1993 May;59(5):1549-54 PubMed
J Mar Biotechnol. 1998 Aug;6(3):193-7 PubMed
Int J Syst Evol Microbiol. 2001 Nov;51(Pt 6):2031-6 PubMed
Antonie Van Leeuwenhoek. 1976;42(3):277-86 PubMed
Can J Microbiol. 1998 Jul;44(7):637-45 PubMed
Biosci Biotechnol Biochem. 2006 Mar;70(3):722-5 PubMed
Appl Environ Microbiol. 1992 Dec;58(12):4060-3 PubMed
Mol Gen Genet. 1987 Aug;209(1):101-9 PubMed
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