Sphingobium czechense sp. nov., isolated from a hexachlorocyclohexane dump site
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- DNA, Bacterial genetics MeSH
- Phylogeny * MeSH
- Hexachlorocyclohexane * MeSH
- Nucleic Acid Hybridization MeSH
- Soil Pollutants * MeSH
- Fatty Acids analysis MeSH
- Molecular Sequence Data MeSH
- Soil Microbiology * MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Sequence Analysis, DNA MeSH
- Spermidine analysis MeSH
- Sphingomonadaceae classification genetics isolation & purification MeSH
- Bacterial Typing Techniques MeSH
- Ubiquinone analysis MeSH
- Base Composition MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- DNA, Bacterial MeSH
- Hexachlorocyclohexane * MeSH
- Soil Pollutants * MeSH
- Fatty Acids MeSH
- RNA, Ribosomal, 16S MeSH
- Spermidine MeSH
- Ubiquinone MeSH
- Ubiquinone Q2 MeSH Browser
A yellow-pigmented bacterial strain, designated LL01(T), was isolated from hexachlorocyclohexane (HCH)-contaminated soil at Spolana Neratovice, a former Czech producer of lindane. A neighbour-joining tree based on 16S rRNA gene sequences showed that strain LL01(T) occupied a distinct phylogenetic position in the Sphingobium cluster, showing highest similarity to Sphingobium rhizovicinum CC-FH12-1(T) (98.5 %). The DNA G+C content of strain LL01(T) was 66.1 mol%. The predominant respiratory pigment was ubiquinone Q-10. The polar lipid profile of strain LL01(T) also corresponded to those reported for other Sphingobium species (phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidyldimethylethanolamine, sphingoglycolipids), supporting its identification as a member of the genus Sphingobium. Spermidine was the major polyamine observed. The results obtained from DNA-DNA hybridization and biochemical and physiological tests clearly distinguished strain LL01(T) from closely related species of the genus Sphingobium. Therefore, strain LL01(T) represents a novel species of the genus Sphingobium, for which the name Sphingobium czechense sp. nov. is proposed (type strain LL01(T) = CCM 7979(T) = DSM 25410(T)).
IMTECH Institute of Microbial Technology Sector 39A Chandigarh 160036 India
Molecular Biology Laboratory Department of Zoology University of Delhi Delhi 110007 India
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