Sperm cells induce distinct cytokine response in peripheral mononuclear cells from infertile women with serum anti-sperm antibodies
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
22952917
PubMed Central
PMC3432089
DOI
10.1371/journal.pone.0044172
PII: PONE-D-12-13465
Knihovny.cz E-resources
- MeSH
- Immune Sera blood immunology MeSH
- Cell Extracts MeSH
- Cytokines metabolism MeSH
- Adult MeSH
- Fertility immunology MeSH
- Leukocytes, Mononuclear metabolism MeSH
- Humans MeSH
- Antibodies blood immunology MeSH
- Spermatozoa immunology MeSH
- Infertility, Female blood immunology MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Immune Sera MeSH
- Cell Extracts MeSH
- Cytokines MeSH
- Antibodies MeSH
BACKGROUND AND AIMS: Anti-sperm antibodies in can markedly reduce the likelihood of natural conception. The etiology of this anti-sperm immunity in human females is unknown. We compared the cytokine response of peripheral blood mononuclear cells (PBMCs) from infertile patients with or without anti-sperm antibodies (ASA) and fertile women. METHODOLOGY/PRINCIPAL FINDINGS: We cultivated the PBMCs together with sperm antigens (whole cells or cell lysate), and screened the supernatants for 40 cytokines by antibody array. When stimulated with whole sperm cells, the PBMCs from patients with ASA produce less IL-3, IL-11, IL-13, ICAM-1, GCSF and more IL-2, IL-4 and IL-12p70 as compared to healthy women. PBMCs from patients with ASA produce typically less IL-13, IL-7, IL-17 and MIG, and more MIP-1β and IL-8, as compared to PBMCs from patients without ASA. In response to sperm cell lysate, PBMCs from infertile women without ASA respond initially by increase in production of growth factors (GCSF, GM-CSF and PDGF-BB) followed by increase in chemokines (e.g. IL-8, MCP-1 and MIP-1β). CONCLUSIONS: Cellular immune responses to sperm antigens, measured by production of cytokines, differ among infertile women with ASA, infertile women without ASA and healthy women. This difference could play an important role in the initial steps of the infertility pathogenesis.
See more in PubMed
Czerkinsky C, Prince SJ, Michalek SM, Jackson S, Russell MW, et al. (1987) IgA antibody-producing cells in peripheral blood after antigen ingestion: evidence for a common mucosal immune system in humans. Proc Natl Acad Sci U S A 84: 2449–2453. PubMed PMC
McDermott MR, Bienenstock J (1979) Evidence for a common mucosal immunologic system. I. Migration of B immunoblasts into intestinal, respiratory, and genital tissues. J Immunol 122: 1892–1898. PubMed
Hill JA, Haimovici F, Anderson DJ (1987) Products of activated lymphocytes and macrophages inhibit mouse embryo development in vitro. J Immunol 139: 2250–2254. PubMed
Naz RK, Mehta K (1989) Cell-mediated immune responses to sperm antigens: effects on mouse sperm and embryos. Biol Reprod 41: 533–542. PubMed
Chaouat G, Menu E, Clark DA, Dy M, Minkowski M, et al. (1990) Control of fetal survival in CBA x DBA/2 mice by lymphokine therapy. J Reprod Fertil 89: 447–458. PubMed
Chaouat G, Ledee-Bataille N, Dubanchet S, Zourbas S, Sandra O, et al. (2004) TH1/TH2 paradigm in pregnancy: paradigm lost? Cytokines in pregnancy/early abortion: reexamining the TH1/TH2 paradigm. Int Arch Allergy Immunol 134: 93–119. PubMed
Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, et al. (1985) Measurement of Protein Using Bicinchoninic Acid. Analytical Biochemistry 150: 76–85. PubMed
World Health Organization (1999) WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. Cambridge, UK; New York, NY: Published on behalf of the World Health Organization by Cambridge University Press., 1–128.
Hjort T, Meinertz H (1988) Anti-sperm antibodies and immune subfertility. Hum Reprod 3: 59–62. PubMed
Kamada M, Maegawa M, Yan YC, Koide SS, Aono T (1999) Antisperm antibody: a monkey wrench in conception/magic bullet of contraception? J Med Invest 46: 19–28. PubMed
Ulcova-Gallova Z, Bouse V, Rokyta Z, Krizanovska K (2000) Effect of corticosteroids on sperm antibody concentration in different biological fluids and on pregnancy outcome in immunologic infertility. Zentralbl Gynakol 122: 495–499. PubMed
Ulcova-Gallova Z (2010) Immunological and physicochemical properties of cervical ovulatory mucus. J Reprod Immunol 86: 115–121. PubMed
McShane PM, Schiff I, Trentham DE (1985) Cellular immunity to sperm in infertile women. JAMA 253: 3555. PubMed
Chaouat G, Dubanchet S, Ledee N (2007) Cytokines: Important for implantation? J Assist Reprod Genet 24: 491–505. PubMed PMC
Robertson SA (2000) Control of the immunological environment of the uterus. Rev Reprod 5: 164–174. PubMed
Wira CR, Crane-Godreau MA, Grant KS (2005) Endocrine Regulation of the Mucosal Immune System in the Female Reproductive Tract. In: Mestecky J, Bienenstock J, Lamm ME, McGhee J, Strober W, et al.., editors. Mucosal Immunology. 3 ed. Amsterdam: Elsevier–Academic Press. 1661–1676.
Paul WE (1989) Pleiotropy and redundancy: T cell-derived lymphokines in the immune response. Cell 57: 521–524. PubMed
Koumantaki Y, Matalliotakis I, Sifakis S, Kyriakou D, Neonaki M, et al. (2001) Detection of interleukin-6, interleukin-8, and interleukin-11 in plasma from women with spontaneous abortion. Eur J Obstet Gynecol Reprod Biol 98: 66–71. PubMed
Staunton DE, Marlin SD, Stratowa C, Dustin ML, Springer TA (1988) Primary structure of ICAM-1 demonstrates interaction between members of the immunoglobulin and integrin supergene families. Cell 52: 925–933. PubMed
Somigliana E, Vigano P, Gaffuri B, Guarneri D, Busacca M, et al. (1996) Human endometrial stromal cells as a source of soluble intercellular adhesion molecule (ICAM)-1 molecules. Hum Reprod 11: 1190–1194. PubMed
Hammadeh ME, Fischer-Hammadeh C, Hoffmeister H, Huebner U, Georg T, et al. (2003) Fibroblast growth factor (FGF), intracellular adhesion molecule (sICAM-1) level in serum and follicular fluid of infertile women with polycystic ovarian syndrome, endometriosis and tubal damage, and their effect on ICSI outcome. Am J Reprod Immunol 50: 124–130. PubMed
Ulcova-Gallova Z, Mukensnabl P, Hadravska S, Kyselova V, Slechtova J, et al. (2005) Antibodies against annexin V and prothrombin, their correlation with other anti-phospholipid antibodies in recurrent pregnancy loss. J Reprod & Contraception 16: 35–46.
Minty A, Chalon P, Derocq JM, Dumont X, Guillemot JC, et al. (1993) Interleukin-13 is a new human lymphokine regulating inflammatory and immune responses. Nature 362: 248–250. PubMed
Gallinelli A, Chiossi G, Giannella L, Marsella T, Genazzani AD, et al. (2004) Different concentrations of interleukins in the peritoneal fluid of women with endometriosis: relationships with lymphocyte subsets. Gynecol Endocrinol 18: 144–151. PubMed
Fukushi J, Ono M, Morikawa W, Iwamoto Y, Kuwano M (2000) The activity of soluble VCAM-1 in angiogenesis stimulated by IL-4 and IL-13. J Immunol 165: 2818–2823. PubMed
Welch PA, Namen AE, Goodwin RG, Armitage R, Cooper MD (1989) Human IL-7: a novel T cell growth factor. J Immunol 143: 3562–3567. PubMed
Ostanin AA, Aizikovich BI, Aizikovich IV, Kozhin AY, Chernykh ER (2007) Role of cytokines in the regulation of reproductive function. Bull Exp Biol Med 143: 75–79. PubMed
Friberg J (1974) Immunological studies on sperm-agglutinating sera from women. Acta Obstet Gynecol Scand Suppl 36: 31–42. PubMed
Kverka M, Burianova J, Lodinova-Zadnikova R, Kocourkova I, Cinova J, et al. (2007) Cytokine profiling in human colostrum and milk by protein array. Clin Chem 53: 955–962. PubMed
Tusher VG, Tibshirani R, Chu G (2001) Significance analysis of microarrays applied to the ionizing radiation response. Proc Natl Acad Sci U S A 98: 5116–5121. PubMed PMC
Zhao Y, Karypis G (2003) Clustering in life sciences. Methods Mol Biol 224: 183–218. PubMed