The entire plasmid content of a multidrug-resistant, CTX-M-15-producing Klebsiella pneumoniae ST416 clone was investigated. Two FII(K) plasmids, pKDO1 (127 kb) and pKPN-CZ (207 kb), were identified and found to carry a formidable set of genes conferring resistance to toxic compounds, metals, and antimicrobial drugs and exhibiting novel features putatively associated with adaptation and fitness of the bacterium in the human host.

Department of Biology and Wildlife Diseases Faculty of Veterinary Hygiene and Ecology University of Veterinary and Pharmaceutical Sciences Brno Brno Czech Republic

Zobrazit více v PubMed

Ruiz E, Rojo-Bezares B, Sáenz Y, Olarte I, Esteban I, Rocha-Gracia R, Zarazaga M, Torres C. 2010. Outbreak caused by a multi-resistant Klebsiella pneumoniae strain of new sequence type ST341 carrying new genetic environments of aac(6′)-Ib-cr and qnrS1 genes in a neonatal intensive care unit in Spain. Int. J. Med. Microbiol. 300:464–469 PubMed

Sandegren L, Linkevicius M, Lytsy B, Melhus Å, Andersson DI. 2012. Transfer of an Escherichia coli ST131 multiresistance cassette has created a Klebsiella pneumoniae-specific plasmid associated with a major nosocomial outbreak. J. Antimicrob. Chemother. 67:74–83 PubMed

Cantón R, González-Alba JM, Galán JC. 2012. CTX-M enzymes: origin and diffusion. Front. Microbiol. 3:110. PubMed PMC

Carattoli A. 2009. Resistance plasmid families in Enterobacteriaceae. Antimicrob. Agents Chemother. 53:2227–2238 PubMed PMC

Madec JY, Poirel L, Saras E, Gourguechon A, Girlich D, Nordmann P, Haenni M. 2012. Non-ST131 Escherichia coli from cattle harbouring human-like blaCTX-M-15-carrying plasmids. J. Antimicrob. Chemother. 67:578–581 PubMed

Johnson TJ, Nolan LK. 2009. Pathogenomics of the virulence plasmids of Escherichia coli. Microbiol. Mol. Biol. Rev. 73:750–754 PubMed PMC

Dolejska M, Brhelova E, Dobiasova H, Krivdova J, Jurnakova J, Sevcikova A, Dubska L, Literak I, Cizek A, Vavrina M, Kutnikova L, Sterba J. Dissemination of IncFIIK-type plasmids in multiresistant CTX-M-15-producing Enterobacteriaceae isolates from children in hospital paediatric oncology wards. Int. J. Antimicrob. Agents, in press PubMed

Villa L, García-Fernández A, Fortini D, Carattoli A. 2010. Replicon sequence typing of IncF plasmids carrying virulence and resistance determinants. J. Antimicrob. Chemother. 65:2518–2529 PubMed

García-Fernández A, Villa L, Carta C, Venditti C, Giordano A, Venditti M, Mancini C, Carattoli A. 2012. Klebsiella pneumoniae ST258 producing KPC-3 identified in Italy carries novel plasmids and OmpK36/OmpK35 porin variants. Antimicrob. Agents Chemother. 56:2143–2145 PubMed PMC

Boyd DA, Tyler S, Christianson S, McGeer A, Muller MP, Willey BM, Bryce E, Gardam M, Nordmann P, Mulvey MR. 2004. Complete nucleotide sequence of a 92-kilobase plasmid harboring the CTX-M-15 extended-spectrum beta-lactamase involved in an outbreak in long-term-care facilities in Toronto, Canada. Antimicrob. Agents Chemother. 48:3758–3764 PubMed PMC

Woodford N, Carattoli A, Karisik E, Underwood A, Ellington MJ, Livermore DM. 2009. Complete nucleotide sequences of plasmids pEK204, pEK499, and pEK516, encoding CTX-M enzymes in three major Escherichia coli lineages from the United Kingdom, all belonging to the international O25:H4-ST131 clone. Antimicrob. Agents Chemother. 53:4472–4482 PubMed PMC

Hiron A, Posteraro B, Carrière M, Remy L, Delporte C, La Sorda M, Sanguinetti M, Juillard V, Borezée-Durant E. 2010. A nickel ABC-transporter of Staphylococcus aureus is involved in urinary tract infection. Mol. Microbiol. 77:1246–1260 PubMed

Samen U, Gottschalk B, Eikmanns BJ, Reinscheid DJ. 2004. Relevance of peptide uptake systems to the physiology and virulence of Streptococcus agalactiae. J. Bacteriol. 186:1398–1408 PubMed PMC

Gerlach GF, Allen BL, Clegg S. 1989. Type 3 fimbriae among enterobacteria and the ability of spermidine to inhibit Mr/K hemagglutination. Infect. Immun. 57:219–224 PubMed PMC

Norman A, Hansen LH, She Q, Sørensen SJ. 2008. Nucleotide sequence of pOLA52: a conjugative IncX1 plasmid from Escherichia coli which enables biofilm formation and multidrug efflux. Plasmid 60:59–74 PubMed

Ong CL, Beatson SA, McEwan AG, Schembri MA. 2009. Conjugative plasmid transfer and adhesion dynamics in an Escherichia coli biofilm. Appl. Environ. Microbiol. 75:6783–6791 PubMed PMC

Bojer MS, Struve C, Ingmer H, Hansen DS, Krogfelt KA. 2010. Heat resistance mediated by a new plasmid encoded Clp ATPase, ClpK, as a possible novel mechanism for nosocomial persistence of Klebsiella pneumoniae. PLoS One 5:e15467 doi:10.1371/journal.pone.0015467 PubMed DOI PMC

Complete Nucleotide Sequences of Two VIM-1-Encoding Plasmids from Klebsiella pneumoniae and Leclercia adecarboxylata Isolates of Czech Origin

Zobrazit více v PubMed

GENBANK
JX424423, JX424424