Two Gram-positive, catalase-negative bacterial strains were isolated from the cloaca of common kingfishers (Alcedo atthis). Repetitive sequence-based PCR fingerprinting using the (GTG)5 primer grouped these isolates into a single cluster separated from all known enterococcal species. The two strains revealed identical 16S rRNA gene sequences placing them within the genus Enterococcus with Enterococcus aquimarinus LMG 16607(T) as the closest relative (97.14 % similarity). Further taxonomic investigation using sequencing of the genes for the superoxide dismutase (sodA), phenylalanyl-tRNA synthase alpha subunit (pheS) and the RNA polymerase alpha subunit (rpoA) as well as application of whole-cell protein fingerprinting, automated ribotyping and extensive phenotyping confirmed that both strains belong to the same species. Based on data from this polyphasic study, these strains represent a novel species of the genus Enterococcus, for which the name Enterococcus alcedinis sp. nov. is proposed. The type strain is L34(T) (= CCM 8433(T) = LMG 27164(T)).

CEITEC VFU University of Veterinary and Pharmaceutical Sciences Palackého 1 3 612 42 Brno Czech Republic

Czech Collection of Microorganisms Department of Experimental Biology Faculty of Science Masaryk University Tvrdého 14 602 00 Brno Czech Republic

Department of Biology and Wildlife Diseases Faculty of Veterinary Hygiene and Ecology University of Veterinary and Pharmaceutical Sciences Palackého 1 3 612 42 Brno Czech Republic

Department of Diagnostic Medicine and Pathobiology College of Veterinary Medicine Kansas State University Manhattan KS 66506 USA

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JX948095, JX948096, JX948097, JX948098, JX948099, JX948100, JX948101, JX948102