Recurrent pregnancy loss, plasminogen activator inhibitor-1 (-675) 4G/5G polymorphism and antiphospholipid antibodies in Czech women
Language English Country Denmark Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
23445116
DOI
10.1111/aji.12099
Knihovny.cz E-resources
- MeSH
- Antibodies, Antiphospholipid blood MeSH
- White People genetics MeSH
- Adult MeSH
- Gene Frequency MeSH
- Genotype MeSH
- Abortion, Habitual blood genetics immunology MeSH
- Plasminogen Activator Inhibitor 1 genetics MeSH
- Humans MeSH
- Polymorphism, Genetic MeSH
- Pregnancy MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Pregnancy MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- Antibodies, Antiphospholipid MeSH
- Plasminogen Activator Inhibitor 1 MeSH
PROBLEM: This study compares the frequencies of plasminogen activator inhibitor-1 (-675) 4G/5G polymorphism and its relationship with eight antiphospholipid antibodies (aPLs) in serum of 157 patients with repeated pregnancy loss (RPL). METHOD OF STUDY: PAI-1 (-675) 4G/5G polymorphism was determined using standard PCR-RFLP method. Enzyme-linked immunosorbent assay was used for the detection of aPLs against ph-serine, ph-ethanolamine, ph-inositol, ph-DL-glycerol, phosphatidic acid, annexin V, cardiolipin, and beta2-GPI. Allelic frequency and distribution of genotypes were calculated. The prevalence of the risk conferring 4G allele and 4G/4G homozygous genotype in patients and controls was compared, and the correlation between aPLs positivity and PAI-1 4G/4G genotype was tested by chi-square test. RESULTS: Statistically highly significant correlation between RPL and PAI-1 (-675) 4G/4G genotype was found. No correlation between PAI-1 (-675) 4G/5G polymorphism and the presence of antiphospholipid antibodies in RPL patients was observed. CONCLUSIONS: PAI-1 (-675) 4G/4G homozygous genotype increases the risk of RPL independently from the aPLs positivity.
References provided by Crossref.org
The role of antiphospholipid antibodies (aPls) in infertile women: the long-lasting experience
