The antimicrobial action of histones in the reproductive tract of cow
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24361880
DOI
10.1016/j.bbrc.2013.12.077
PII: S0006-291X(13)02148-7
Knihovny.cz E-resources
- Keywords
- Antimicrobial properties, Bovine reproductive tract, Extracellular histones, Histone-like proteins,
- MeSH
- Anti-Infective Agents metabolism MeSH
- Dialysis MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- Histones chemistry metabolism MeSH
- Mass Spectrometry MeSH
- Cattle MeSH
- Body Fluids metabolism MeSH
- Oviducts metabolism MeSH
- Genitalia, Female metabolism MeSH
- Animals MeSH
- Check Tag
- Cattle MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Anti-Infective Agents MeSH
- Histones MeSH
An infection of any part of female reproductive tract can severely interfere with fertility and reproduction. The fluids and epithelium from the lumen of the female reproductive tract (uterus, oviduct and ovarian follicle) are a known source of antimicrobial action in several species. In this study, we compared the antimicrobial properties of fluids from the reproductive tract of a cow. After removal of small molecules, we demonstrated that there is an antimicrobial activity connected with a fraction of compounds with a molecular mass range between 3500 and 30,000. The most probable candidates responsible for the observed antimicrobial effect were subsequently identified by mass spectroscopy as histones H2A type 2-C, H2B type 1-K, H3.3, and H4. The antimicrobial role of histone H2B was further confirmed by using an antibody against this histone.
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