Manganese-induced oxidative stress in two ontogenetic stages of chamomile and amelioration by nitric oxide
Language English Country Ireland Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24388509
DOI
10.1016/j.plantsci.2013.10.015
PII: S0168-9452(13)00232-X
Knihovny.cz E-resources
- Keywords
- Fluorescence microscopy, Heavy metals, Mineral nutrients, Ontogenesis, Reactive oxygen species,
- MeSH
- Antioxidants metabolism MeSH
- Nitric Oxide Donors pharmacology MeSH
- Microscopy, Fluorescence methods MeSH
- Adaptation, Physiological * MeSH
- Manganese adverse effects metabolism MeSH
- Matricaria drug effects growth & development metabolism MeSH
- Nitroprusside pharmacology MeSH
- Nitric Oxide metabolism pharmacology MeSH
- Oxidative Stress * MeSH
- Hydrogen Peroxide metabolism MeSH
- Peroxidases metabolism MeSH
- Reactive Oxygen Species metabolism MeSH
- Seedlings drug effects growth & development metabolism MeSH
- Sulfhydryl Compounds metabolism MeSH
- Superoxides metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antioxidants MeSH
- Nitric Oxide Donors MeSH
- Manganese MeSH
- Nitroprusside MeSH
- Nitric Oxide MeSH
- Hydrogen Peroxide MeSH
- Peroxidases MeSH
- Reactive Oxygen Species MeSH
- Sulfhydryl Compounds MeSH
- Superoxides MeSH
Impact of manganese (Mn(2+)) excess (100, 500 and 1000 μM over 7 days) on two ontogenetic stages (7-week-old plants and 7-day-old seedlings) of Matricaria chamomilla was compared. Mn excess depressed growth of seedlings (but not germination) and stimulated oxidative stress (ROS and lipid peroxidation) in both plants and seedlings. Growth inhibition could be evoked by higher Mn uptake and higher translocation factor in seedlings than in plants. Total thiols staining revealed elevation in almost all treatments. In 7-week-old plants, activity of peroxidases increased slightly and rather decreased under high Mn doses. Superoxide rather than hydrogen peroxide contributed to visualized ROS presence. Fluorescence of nitric oxide (NO) showed stimulation in plants but decrease in seedlings. Impact of exogenous nitric oxide donor (sodium nitroprusside/SNP) was therefore tested and results showed amelioration of 1000 μM Mn-induced oxidative stress in seedlings (decrease in H2O2 and increase in NO content while antioxidative enzyme activities were variably affected) concomitantly with depleted Mn accumulation. It is concluded that NO participates in tolerance to Mn excess but negative effects of the highest SNP dose were also observed. Extensive fluorescence microscopy is also explanatively discussed.
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