Identification of Streptomyces coelicolor M145 genomic region involved in biosynthesis of teichulosonic acid-cell wall glycopolymer
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Polysaccharides, Bacterial biosynthesis MeSH
- Cell Wall genetics metabolism MeSH
- Data Mining MeSH
- DNA, Bacterial genetics MeSH
- Mutagenesis, Insertional MeSH
- Cloning, Molecular MeSH
- Sugar Acids metabolism MeSH
- Magnetic Resonance Spectroscopy MeSH
- Streptomyces coelicolor genetics metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 3-deoxyglycero-galacto-nonulosonic acid MeSH Browser
- Polysaccharides, Bacterial MeSH
- DNA, Bacterial MeSH
- Sugar Acids MeSH
The cell wall of the model actinomycete Streptomyces coelicolor M145 has recently been shown to contain the novel glycopolymer teichulosonic acid. The major building block of this polymer is 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (Kdn), suggesting initial clues about the genetic control of biosynthesis of this cell wall component. Here, through genome mining and gene knockouts, we demonstrate that the sco4879-sco4882 genomic region of S. coelicolor M145 is necessary for biosynthesis of teichulosonic acid. Specifically, mutants carrying individual knockouts of sco4879, sco4880 and sco4881 genes do not produce Kdn-containing glycopolymer and instead accumulate the minor cell wall component poly(diglycosyl 1-phosphate). Our studies provide evidence that this region is at least partly responsible for biosynthesis of Kdn, whereas flanking genes might control the other steps of teichulosonic acid formation.
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