OBJECTIVE: To present our experience with ultrasound-guided minimally invasive bioptic techniques in gynecologic oncology. SUBJECT: Original paper. SETTING: Gynecological Oncology Center, Department of Obstetrics and Gynecology, Charles University, First Faculty of Medicine and General Faculty Hospital, Prague. SUBJECT AND METHOD: Minimally invasive ultrasound-guided biopsy may be done in patients with primary inoperable disease, suspicious recurrence of gynecological or non-gynecological malignancy or in case of diagnostic uncertainty of pelvic tumor origin. CONCLUSION: Ultrasound-guided minimally invasive bio-psy represents an accessible and relatively simple method useful in many indications, not only in gynecologic oncology.