Bacteria of the genus Rickettsia in ticks (Acari: Ixodidae) collected from birds in Costa Rica
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
25869035
DOI
10.1016/j.ttbdis.2015.03.016
PII: S1877-959X(15)00053-9
Knihovny.cz E-zdroje
- Klíčová slova
- Amblyomma, Birds, Costa Rica, Ixodes, Rickettsia, Ticks,
- MeSH
- divoká zvířata parazitologie MeSH
- infestace klíšťaty parazitologie veterinární MeSH
- Ixodidae klasifikace genetika růst a vývoj mikrobiologie MeSH
- larva klasifikace genetika růst a vývoj mikrobiologie MeSH
- nemoci ptáků parazitologie MeSH
- ptáci MeSH
- Rickettsia klasifikace genetika izolace a purifikace MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Kostarika MeSH
The aim of this study was to document the presence of Rickettsia spp. in ticks parasitizing wild birds in Costa Rica. Birds were trapped at seven locations in Costa Rica during 2004, 2009, and 2010; then visually examined for the presence of ticks. Ticks were identified, and part of them was tested individually for the presence of Rickettsia spp. by polymerase chain reaction (PCR) using primers targeting fragments of the rickettsial genes gltA and ompA. PCR products were DNA-sequenced and analyzed in BLAST to determine similarities with previously reported rickettsial agents. A total of 1878 birds were examined, from which 163 birds (9%) were infested with 388 ticks of the genera Amblyomma and Ixodes. The following Amblyomma (in decreasing order of abundance) were found in immature stages (larvae and nymphs): Amblyomma longirostre, Amblyomma calcaratum, Amblyomma coelebs, Amblyomma sabanerae, Amblyomma varium, Amblyomma maculatum, and Amblyomma ovale. Ixodes ticks were represented by Ixodes minor and two unclassified species, designated here as Ixodes sp. genotype I, and Ixodes sp. genotype II. Twelve of 24 tested A. longirostre ticks were found to be infected with 'Candidatus Rickettsia amblyommii', and 2 of 4 A. sabanerae were found to be infected with Rickettsia bellii. Eight of 10 larval Ixodes minor were infected with an endosymbiont (a novel Rickettsia sp. agent) genetically related to the Ixodes scapularis endosymbiont. No rickettsial DNA was found in A. calcaratum, A. coelebs, A. maculatum, A. ovale, A. varium, Ixodes sp. I, and Ixodes sp. II. We report the occurrence of I. minor in Costa Rica for the first time and a number of new bird host-tick associations. Moreover, 'Candidatus R. amblyommii' and R. bellii were found in A. longirostre and A. sabanerae, respectively, in Costa Rica for the first time.
Institute Adolfo Lutz Center of Immunology Immunoproteomic Lab São Paulo Brazil
Institute of Vertebrate Biology Academy of Sciences of the Czech Republic v v i Brno Czech Republic
Laboratory of Hantaviruses and Rickettsioses Instituto Oswaldo Cruz FIOCRUZ Rio de Janeiro Brazil
Citace poskytuje Crossref.org