Quantification of nanomaterial bioconjugation based on electrophoretic mobility shift
Language English Country Germany Media print
Document type Journal Article
- Keywords
- DNA, Electrophoretic mobility shift assay, Protein, Quantum dots,
- MeSH
- DNA analysis chemistry MeSH
- Quantum Dots analysis chemistry MeSH
- Nanoconjugates analysis chemistry MeSH
- Electrophoretic Mobility Shift Assay methods MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- DNA MeSH
- Nanoconjugates MeSH
Electrophoretic mobility shift assay (EMSA) is a well-established technique to monitor interactions between biomolecules particularly DNA and proteins. Even though numerous variations of this method have been presented, challenges in the form of detection sensitivity and/or variations in the stability of the formed complex still remain. With advances in the area of nanomaterials improvements in EMSA have been also suggested. Recently, Zhang and Wang (Electrophoresis 2015, 36, 1011-1015) presented electrophoretic mobility shift method for determination of number of DNA molecules conjugated to quantum dots (QDs), which was further utilized for calculation of enzymatic activity, sequence specific DNA detection, and neutral molecule quantification.
Central European Institute of Technology Brno University of Technology Brno Czech Republic
Department of Chemistry and Biochemistry Mendel University in Brno Brno Czech Republic
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