Chorioamniotic membrane senescence: a signal for parturition?
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26025293
DOI
10.1016/j.ajog.2015.05.041
PII: S0002-9378(15)00517-7
Knihovny.cz E-resources
- Keywords
- aging, delivery, fetal signals, labor, pregnancy, premature birth, senescence-associated secretory phenotype, sterile inflammation, β-galactosidase,
- MeSH
- Amnion cytology metabolism ultrastructure MeSH
- beta-Galactosidase metabolism MeSH
- Chemokine CCL11 metabolism MeSH
- Chorion cytology metabolism ultrastructure MeSH
- Adult MeSH
- Granulocyte-Macrophage Colony-Stimulating Factor metabolism MeSH
- Interleukin-6 metabolism MeSH
- Interleukin-8 metabolism MeSH
- Humans MeSH
- Fas Ligand Protein metabolism MeSH
- Intercellular Adhesion Molecule-1 metabolism MeSH
- Mitochondria ultrastructure MeSH
- Young Adult MeSH
- Osteoprotegerin metabolism MeSH
- Amniotic Fluid cytology metabolism MeSH
- Term Birth metabolism MeSH
- Labor, Obstetric metabolism MeSH
- Cross-Sectional Studies MeSH
- Cellular Senescence * MeSH
- Case-Control Studies MeSH
- Pregnancy MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Young Adult MeSH
- Pregnancy MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- beta-Galactosidase MeSH
- CCL11 protein, human MeSH Browser
- Chemokine CCL11 MeSH
- CXCL8 protein, human MeSH Browser
- Granulocyte-Macrophage Colony-Stimulating Factor MeSH
- IL6 protein, human MeSH Browser
- Interleukin-6 MeSH
- Interleukin-8 MeSH
- Fas Ligand Protein MeSH
- Intercellular Adhesion Molecule-1 MeSH
- Osteoprotegerin MeSH
- TNFRSF11B protein, human MeSH Browser
OBJECTIVE: Senescence is an important biological phenomenon involved in both physiologic and pathologic processes. We propose that chorioamniotic membrane senescence is a mechanism associated with human parturition. The present study was conducted to explore the association between senescence and normal term parturition by examining the morphologic and biochemical evidences in chorioamniotic membranes. STUDY DESIGN: Chorioamniotic membranes were collected from normal term deliveries; group 1: term labor and group 2: term, not in labor. Senescence-related morphologic changes were determined by transmission electron microscopy and biochemical changes were studied by senescence-associated (SA) β-galactosidase staining. Amniotic fluid samples collected from both term labor and term not in labor were analyzed for 14 SA secretory phenotype (SASP) markers. RESULTS: Morphologic evidence of cellular senescence (enlarged cells and organelles) and a higher number of SA β-galactosidase-stained amnion and chorion cells were observed in chorioamniotic membranes obtained from women in labor at term, when compared to term not in labor. The concentration of proinflammatory SASP markers (granulocyte macrophage colony-stimulating factor, interleukin-6 and -8) was significantly higher in the amniotic fluid of women in labor at term than women not in labor. In contrast, SASP factors that protect against cell death (eotaxin-1, soluble Fas ligand, osteoprotegerin, and intercellular adhesion molecule-1) were significantly lower in the amniotic fluid samples from term labor. CONCLUSION: Morphologic and biochemical features of senescence were more frequent in chorioamniotic membranes from women who experienced term labor. Senescence of chorioamniotic membranes were also associated with amniotic fluid SASP markers.
Department of Obstetrics and Gynecology Charles University Hradec Kralove Czech Republic
Department of Pathology University of Texas Medical Branch Galveston TX
Electron Microscopy Core Laboratory University of Texas Medical Branch Galveston TX
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