Mitochondrial ATP synthase, ADP/ATP translocase (ANT), and inorganic phosphate carrier (PiC) are supposed to form a supercomplex called ATP synthasome. Our protein and transcript analysis of rat tissues indicates that the expression of ANT and PiC is transcriptionally controlled in accordance with the biogenesis of ATP synthase. In contrast, the content of ANT and PiC is increased in ATP synthase deficient patients' fibroblasts, likely due to a post-transcriptional adaptive mechanism. A structural analysis of rat heart mitochondria by immunoprecipitation, blue native/SDS electrophoresis, immunodetection and MS analysis revealed the presence of ATP synthasome. However, the majority of PiC and especially ANT did not associate with ATP synthase, suggesting that most of PiC, ANT and ATP synthase exist as separate entities.

Department of Bioenergetics Institute of Physiology of the Czech Academy of Sciences Vídeňská 1083 14220 Prague Czech Republic

Department of Bioenergetics Institute of Physiology of the Czech Academy of Sciences Vídeňská 1083 14220 Prague Czech Republic; Charles University Prague 1st Faculty of Medicine Kateřinská 32 12108 Prague Czech Republic

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