The 22 amphetamine-derived synthetic drugs (ADSDs), mostly cathinones, were examined by gas chromatography with mass spectrometry using two different derivatization methods with (i) heptafluorobutyric anhydride (HFBA) and (ii) pentafluorobenzoyl chloride (PFBCl). Both developed derivatization approaches were evaluated and compared for urine and serum samples. Extraction procedures proved to give satisfactory results with regard to recoveries and extract purity, even though both derivatization methods reached acceptable sensitivity for the intended use. The derivatization with PFBCl showed better results with respect to retention and response stability, thus the PFBCl method was selected for validation. Calibration curves were linear over the tested concentration range of 20-1,000 ng/mL with the R(2) values ranging from 0.994 to 0.998. Intra- and interday precisions and accuracies were within 20% for all concentrations in the linear range. The limit of detection was determined to be lower than 2 ng/mL for all 22 analytes. The method proved to be a useful analytical tool in the course of systematic toxicological analysis.

Faculty of Medicine and Dentistry Department of Forensic Medicine and Medical Law Palacký University Olomouc CZ 77509 Czech Republic Faculty Hospital Palacký University Hnevotinska 3 Olomouc CZ 77509 Czech Republic

Regional Centre of Advanced Technologies and Materials Faculty of Science Department of Analytical Chemistry Palacký University 17 Listopadu 12 Olomouc CZ 77146 Czech Republic

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