Immunomodulation with Self-Crosslinked Polyelectrolyte Multilayer-Based Coatings
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Cell Adhesion drug effects MeSH
- Cell Differentiation drug effects MeSH
- Cytokines administration & dosage chemistry MeSH
- Immunomodulation drug effects MeSH
- Cells, Cultured MeSH
- Hyaluronic Acid chemistry MeSH
- Humans MeSH
- Macrophages cytology drug effects metabolism MeSH
- Monocytes cytology drug effects metabolism MeSH
- Polyelectrolytes chemistry MeSH
- Surface Properties MeSH
- Cross-Linking Reagents chemistry MeSH
- Inflammation drug therapy immunology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Cytokines MeSH
- Hyaluronic Acid MeSH
- Polyelectrolytes MeSH
- Cross-Linking Reagents MeSH
This study aims to design an optimal polyelectrolyte multilayer film of poly-l-lysine (PLL) and hyaluronic acid (HA) as an anti-inflammatory cytokine release system in order to decrease the implant failure due to any immune reactions. The chemical modification of the HA with aldehyde moieties allows self-cross-linking of the film and an improvement in the mechanical properties of the film. The cross-linking of the film and the release of immunomodulatory cytokine (IL-4) stimulate the differentiation of primary human monocytes seeded on the films into pro-healing macrophages phenotype. This induces the production of anti-inflammatory cytokines (IL1-RA and CCL18) and the decrease of pro-inflammatory cytokines secreted (IL-12, TNF-α, and IL-1β). Moreover, we demonstrate that cross-linking PLL/HA film using HA-aldehyde is already effective by itself to limit inflammatory processes. Finally, this functionalized self-cross-linked PLL/HA-aldehyde films constitutes an innovative and efficient candidate for immunomodulation of any kind of implants of various architecture and properties.
Contipro a s Dolni Dobrouc 401 561 02 Dolni Dobrouc Czech Republic
INSERM UMR 1121 11 rue Humann 67085 Strasbourg France
Institut Charles Sadron CNRS UPR 22 23 rue du Lœss 67034 Strasbourg France
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