Autochthonous Hepatozoon infection in hunting dogs and foxes from the Czech Republic
Language English Country Germany Media print-electronic
Document type Journal Article
PubMed
27431805
DOI
10.1007/s00436-016-5191-2
PII: 10.1007/s00436-016-5191-2
Knihovny.cz E-resources
- Keywords
- Autochthonous infection, Czech Republic, Dogs, Hepatozoon canis, Red foxes,
- MeSH
- Arachnid Vectors parasitology MeSH
- Eucoccidiida classification genetics isolation & purification MeSH
- Phylogeny MeSH
- Coccidiosis epidemiology parasitology transmission veterinary MeSH
- Foxes parasitology MeSH
- Dog Diseases epidemiology parasitology transmission MeSH
- Dogs MeSH
- Rhipicephalus sanguineus parasitology MeSH
- DNA, Ribosomal genetics MeSH
- Sequence Analysis, DNA veterinary MeSH
- Sequence Alignment veterinary MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Dogs MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Czech Republic epidemiology MeSH
- Names of Substances
- DNA, Ribosomal MeSH
Blood samples from 21 red foxes (Vulpes vulpes) and 8 hunting dogs from the same locality in the Czech Republic were examined for presence of Hepatozoon canis/Hepatozoon sp. The dogs were selected based on their close contact with foxes during fox bolting and because they had not traveled into known endemic areas. Using diagnostic PCR amplifying partial 18S rDNA fragment, Hepatozoon DNA was detected in 20 red foxes (95 %) and 4 dogs (50 %). From 8 positive foxes and 2 positive dogs, we obtained nearly complete 18S rDNA sequences. Phylogenetic analyses of these sequences revealed very low variability. Buffy coat smears from positive dogs were prepared and examined. No Hepatozoon gamonts were found. This study provides the first report of autochthonous infection of H. canis/Hepatozoon in dogs and foxes from the Czech Republic. Our study indirectly demonstrates cross infection between red foxes and dogs and confirms autochthonous infection of Hepatozoon canis in dogs living in a geographic area well outside the range of Rhipicephalus sanguineus sensu lato, which is so far the only known vector of H. canis in Europe.
Department of Virology Veterinary Research Institute 62100 Brno Czech Republic
Military Veterinary Institute Army of the Czech Republic Opavská 29 748 01 Hlučín Czech Republic
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