Transcriptomic response of Arabidopsis thaliana (L.) Heynh. roots to ibuprofen
Language English Country United States Media print
Document type Journal Article
- Keywords
- detoxification, gene expression, metabolism of xenobiotics, microarrays, phytoremediation,
- MeSH
- Arabidopsis metabolism MeSH
- Biodegradation, Environmental MeSH
- Water Pollutants, Chemical toxicity MeSH
- Transcription, Genetic drug effects MeSH
- Ibuprofen pharmacology toxicity MeSH
- Plant Roots MeSH
- Wastewater * MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Water Pollutants, Chemical MeSH
- Ibuprofen MeSH
- Waste Water * MeSH
Surface waters in urban areas are contaminated by ibuprofen (IBP), a popular and extensively used anti-inflammatory drug. In this study, we investigated the transcriptomic response in Arabidopsis thaliana (L.) Heynh. roots with the aim of revealing genes that are potentially involved in IBP detoxification and elucidating the effect of IBP on plants. IBP upregulated 63 and downregulated 38 transcripts (p-value < 0.1, fold change ≥2) after 2-day exposure to a 5-µM (1.03 mg/L) concentration of IBP under hydroponic conditions. Although the IBP concentration used in the experiment was highly relative to the concentrations found in rivers and wastewater, the number of genes with transcriptional changes was relatively low. The upregulation of cytochrome P450s, glutathione S-transferases, and UDP-glycosyltransferases indicates the occurrence of IBP oxidation in the first phase, followed by conjugation with glutathione and sugar in the second detoxification phase. ABC transporters could be involved in the transport of IBP and its metabolites. The identification of genes potentially involved in IBP detoxification could be useful in an IBP phytoremediation approach.
References provided by Crossref.org
Cytokinin at the Crossroads of Abiotic Stress Signalling Pathways
