Our project was aimed at improving a brownfield in the city of Kladno, where an old steel producing facility used to be in operation. Ecological risk is mainly caused by the processing of co-products during coal production (tars, oils). Knowledge of toxicology and environmental aspects can help us protect human health and the environment. Primarily, we focused on soil sampling and identification of pollutants. Results showed that organic contamination on the site is very high. Average concentration of total petroleum carbon in the soil was about 13g/kg DW, which is much more than the maximum allowed concentration. For selection of suitable plant species for phytoremediation at the site, experiments were conducted in a greenhouse. Biomass growth, root morphology, and pigment content in the leaves of Brassica napus var. Opus-C1 and Sorghum×drummondii var. Honey Graze BMR plants were studied. Plant analysis confirmed that polyaromatic hydrocarbons (PAHs) accumulated in the shoots of both plant species. B. napus plants grown on Poldi soil in a greenhouse were able to survive the toxicity of PAHs in soil, and their ability to accumulate PAHs from soil was evident. However, more studies are needed to decide if the plants are usable for phytoremediation of this brownfield.

Laboratory of Plant Biotechnologies Institute of Experimental Botany AS CR Prague Czech Republic

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