Purification of Natural Antibodies Against Tau Protein by Affinity Chromatography
Methods in molecular biology (Clifton, N.J.).
2017 ;
1643 () :
33-44.
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
Persistent link
https://www.medvik.cz/link/pmid28667528
- Keywords
- Human plasma, Immobilization, Immunopurification, Immunoreactivity, Intravenous immunoglobulin, Natural antibodies, Tau protein,
- MeSH
- Autoantibodies immunology isolation & purification MeSH
- Chromatography, Affinity * MeSH
- Immunosorbent Techniques MeSH
- Immunoglobulins, Intravenous MeSH
- Humans MeSH
- tau Proteins immunology MeSH
- Antibodies immunology isolation & purification MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Autoantibodies MeSH
- Immunoglobulins, Intravenous MeSH
- tau Proteins MeSH
- Antibodies MeSH
Natural antibodies are now widely studied for their therapeutical potential. Therefore, their isolation and subsequent characterization is desired. Here, we describe an isolation method for natural anti-tau antibodies from human plasma by utilization of affinity chromatography with epoxy-activated copolymer resin. The evalution of isolation efficiency and avidity of isolated antibodies is decribed by modified indirect ELISA assay.
References provided by Crossref.org
Journal of immunology research.
2019 ;
2019 () :
7406810.
[epub] 20190806
