Isolation and Characterization of CTCs from Patients with Cancer of a Urothelial Origin
Language English Country United States Media print
Document type Journal Article
- Keywords
- Bladder cancer, CTCs, Circulating tumor cells, Cultivation, Gene expression, In vitro, Prostate cancer, Renal,
- MeSH
- Cell Culture Techniques MeSH
- DNA, Neoplasm genetics isolation & purification MeSH
- Humans MeSH
- DNA Mutational Analysis methods MeSH
- Neoplastic Cells, Circulating metabolism pathology MeSH
- Cell Separation * methods MeSH
- Gene Expression Profiling methods MeSH
- Urologic Neoplasms diagnosis genetics metabolism MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- DNA, Neoplasm MeSH
Monitoring of circulating tumor cells' (CTCs) presence has the potential to improve therapeutic management of oncological diseases at an early stage and also to identify patients with increased risk of tumor progression or recurrence before the onset of clinically detected metastasis. Here we describe a new simplified efficient methodology for the separation and in vitro culturing of viable CTCs from peripheral blood by size-based filtration (MetaCell®). The isolation protocol yields preferentially cells bigger than 8 μm enabling further cytomorphological and molecular analysis.
References provided by Crossref.org
