A polyhistidine tag (His-tag) present on Chlorobaculum tepidum reaction centers (RCs) was used to immobilize photosynthetic complexes on a silver nanowire (AgNW) modified with nickel-chelating nitrilo-triacetic acid (Ni-NTA). The optical properties of conjugated nanostructures were studied using wide-field and confocal fluorescence microscopy. Plasmonic enhancement of RCs conjugated to AgNWs was observed as their fluorescence intensity dependence on the excitation wavelength does not follow the excitation spectrum of RC complexes in solution. The strongest effect of plasmonic interactions on the emission intensity of RCs coincides with the absorption spectrum of AgNWs and is observed for excitation into the carotenoid absorption. From the absence of fluorescence decay shortening, we attribute the emission enhancement to increase of absorption in RC complexes.

Baltic Institute of Technology Al Zwycięstwa 96 98 Gdynia Poland

Department of Chemical Physics and Optics Charles University Ke Karlovu 3 Prague Czech Republic

Department of Physiology and Cellular Biophysics Columbia University Russ Berrie Pavilion 1150 St Nicholas Avenue New York NY 10025 USA

Institute of Molecular Cell and Systems Biology Glasgow Biomedical Research Centre University of Glasgow 120 University Place Glasgow G12 8TA Scotland UK

Institute of Physical Chemistry Polish Academy of Sciences Kasprzaka 44 52 Warsaw Poland

Institute of Physics Faculty of Physics Astronomy and Informatics Nicolaus Copernicus University Grudziadzka 5 Torun Poland

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