GC-MS Metabolomic Profiling of Protic Metabolites Following Heptafluorobutyl Chloroformate Mediated Dispersive Liquid Microextraction Sample Preparation Protocol
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
- Klíčová slova
- Chloroformate derivatization, Dispersive liquid-liquid microextraction, GC-MS, Metabolomic profiling, Quantitative analysis, Urine,
- MeSH
- analýza moči metody MeSH
- biologické markery moč MeSH
- fluorokarbony chemie MeSH
- formiáty chemie MeSH
- lidé MeSH
- metabolomika metody MeSH
- mikroextrakce kapalné fáze metody MeSH
- plynová chromatografie s hmotnostně spektrometrickou detekcí metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- biologické markery MeSH
- fluorokarbony MeSH
- formiáty MeSH
- heptafluorobutyl chloroformate MeSH Prohlížeč
A simple analytical workflow is described for gas chromatographic-mass spectrometry (GC-MS)-based metabolomic profiling of protic metabolites, particularly amino-carboxylic species in biological matrices. The sample preparation is carried out directly in aqueous samples and uses simultaneous in situ heptafluorobutyl chloroformate (HFBCF) derivatization and dispersive liquid-liquid microextraction (DLLME), followed by GC-MS analysis in single-ion monitoring (SIM) mode. The protocol involves ten simple pipetting steps and provides quantitative analysis of 132 metabolites by using two internal standards. A comment on each analytical step and explaining notes are provided with particular attention to the GC-MS analysis of 112 physiological metabolites in human urine.
Citace poskytuje Crossref.org
