An UHPLC-MS/MS Method for Target Profiling of Stress-Related Phytohormones
Methods in molecular biology (Clifton, N.J.).
2018 ;
1778 () :
183-192.
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
Persistent link
https://www.medvik.cz/link/pmid29761439
- Keywords
- Abscisic acid, Auxins, Jasmonates, LC-MS, Liquid chromatography–mass spectrometry, Phytohormones, Salicylic acid, Solid-phase extraction (SPE),
- MeSH
- Cyclopentanes analysis MeSH
- Abscisic Acid analysis MeSH
- Salicylic Acid analysis MeSH
- Indoleacetic Acids analysis MeSH
- Oxylipins analysis MeSH
- Plant Growth Regulators analysis MeSH
- Tandem Mass Spectrometry methods MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Cyclopentanes MeSH
- jasmonic acid MeSH Browser
- Abscisic Acid MeSH
- Salicylic Acid MeSH
- Indoleacetic Acids MeSH
- Oxylipins MeSH
- Plant Growth Regulators MeSH
The methodology described here represents an improved strategy for analysis of a broad range of stress-related plant hormones including jasmonates, salicylic acid, abscisic acid, and auxin metabolites. The method conditions are optimized in order to reduce the background effect of complicated plant matrix, allow effective preconcentration and thus perform highly sensitive profiling of multiple plant hormones by ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS).
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