More than 20 years after the first outbreaks, the phylogenetic picture of PRRSV is still incomplete and full of gaps, especially in regards of PRRSV 1. Due to the exceptional diversity observed at the eastern borders of Europe and the low number of available sequences from Central Eastern European countries, the authors collected and analyzed both recent as well as already submitted sequences comparing them to a large backbone set of available ORF5 sequences representing the full spectrum of PRRSV 1 Subtype 1 diversity to conduct a systematic phylogenetic analysis and reclassification elucidating the diversity of the virus in these countries. Moreover, further analyses of the EUROSTAT data regarding the live pig movement trends revealed their influence of virus diversity and evolution. The results indicate that besides the effect of local, isolated divergent evolution and the use of modified live vaccines, the most important factor influencing a given country's virus diversity is the transboundary movement of live, infected animals.

Arbovirology Bernhard Nocht Institute for Tropical Medicine WHO Collaborating Centre for Arbovirus and Haemorrhagic Fever Reference and Research National Reference Centre for Tropical Infectious Diseases Bernhard Nocht Strasse 74 20359 Hamburg Germany

Department of Medical Microbiology Faculty of Medicine Medical University of Sofia Zdrave Str 2 1431 Sofia Bulgaria

Department of Pathology and Veterinary Diagnostics Faculty of Veterinary Medicine Warsaw University of Life Sciences SGGW ul Nowoursynowska 159c 02 776 Warsaw Poland

Department of Pathology University of Veterinary Medicine István Str 2 H 1076 Budapest Hungary

Faculty of Veterinary Medicine Institute of Infectious Diseases and Microbiology University of Veterinary and Pharmaceutical Sciences Brno 612 42 Brno Czech Republic

National Food Chain Safety Office Animal Health and Animal Welfare Directorate Keleti Károly Str 24 H 1024 Budapest Hungary

National Food Chain Safety Office Veterinary Diagnostic Directorate Tábornok Str 2 H 1149 Budapest Hungary

National Veterinary Research Institute Partyzantów 57 24 100 Pulawy Poland

Scientific Veterinary Institute ''Novi Sad Rumenački put 20 21000 Novi Sad Serbia

The University of Hong Kong 5N 12 Kadoorie Biological Science Building Hong Kong China

University of Ljubljana Veterinary Faculty National Veterinary Institute Gerbičeva 60 1000 Ljubljana Slovenia

University of Zagreb Faculty of Agriculture Department of Animal Science Svetošimunska cesta 25 10000 Zagreb Croatia

Veterinary Diagnostic Laboratory Ostródzka 11 036 Gietrzwałd Poland

Veterinary Institute Zvolen Pod Drahami 918 960 86 Zvolen Slovak Republic

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