Polyhydroxybutyrate production by an extremely halotolerant Halomonas elongata strain isolated from the hypersaline meromictic Fără Fund Lake (Transylvanian Basin, Romania)
Language English Country Great Britain, England Media print-electronic
Document type Journal Article
Grant support
PN-II-ID-PCE-2011-3-0546
Romanian National Authority for Scientific Research, CNCS-UEFISCDI
PN-II-ID-PCE-2011-3-0765
Romanian National Authority for Scientific Research, CNCS-UEFISCDI
PN-III-P4-ID-PCE-2016-0303
Romanian National Authority for Scientific Research, CNCS-UEFISCDI
Core Program, PN18-03 02 01
Ministry of Research and Innovation, MCI
31773.3
Babes-Bolyai University
PubMed
29928771
DOI
10.1111/jam.14029
Knihovny.cz E-resources
- Keywords
- Halomonas sp., green plastics, halotolerant bacteria, high salinity, nutrient limitation, polyhydroxybutyrate,
- MeSH
- Biopolymers biosynthesis MeSH
- Sodium Chloride metabolism MeSH
- Phylogeny MeSH
- Halomonas genetics isolation & purification metabolism MeSH
- Lakes microbiology MeSH
- Polyhydroxyalkanoates biosynthesis chemistry MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Romania MeSH
- Names of Substances
- Biopolymers MeSH
- Sodium Chloride MeSH
- Polyhydroxyalkanoates MeSH
- RNA, Ribosomal, 16S MeSH
AIM: This study aimed at unprecedented physical and chemical evaluation of the 'green plastics' polyhydroxyalkanoates (PHAs), in an extremely halotolerant Halomonas elongata strain 2FF under high-salt concentration. METHODS AND RESULTS: The investigated bacterial strain was isolated from the surface water of the hypersaline Fără Fund Lake. The 16S rRNA gene sequence phylogeny and phenotypic analysis indicated that the isolate belonged to H. elongata. PHA inclusions were observed by Sudan Black B, Nile Red staining, and transmission electron microscopy during growth at high salinity (10%, w/v, NaCl) on 1% (w/v) d-glucose. The produced polymer was quantitatively and qualitatively assessed using crotonic acid assay, elemental analysis, Fourier transform infrared and Raman spectroscopies. Additionally, X-ray powder diffraction, 1 H-NMR spectroscopy, and differential scanning calorimetry were applied. The investigations showed that the intracellular polymer was polyhydroxybutyrate (PHB) of which the strain produced up to 40 wt% of total cell dry weight after 48 h. The analysis of phaC gene from the isolated H. elongata strain indicated that the encoded PHA synthase belongs to Class I PHA synthase family. CONCLUSIONS: Overall, our investigations pointed out that the halotolerant H. elongata strain 2FF was capable to produce significant amounts of PHB from d-glucose, and PHAs from various carbon substrates at high-salt concentrations. SIGNIFICANCE AND IMPACT OF THE STUDY: The tested strain showed the ability for significant production of natural, biodegradable polymers under nutrient limitation and hypersaline conditions suggesting its potentiality for further metabolic and molecular investigations towards enhanced biopolymer production. Additionally, this study reports on the unprecedented use of Raman and XPRD techniques to investigate PHAs of an extremely halotolerant bacterium, thus expanding the repertoire of physical methods to study green plastics derived from extremophilic microorganisms.
Department of Biomolecular Physics Faculty of Physics Babeș Bolyai University Cluj Napoca Romania
Electron Microscopy Center Babeș Bolyai University Cluj Napoca Romania
INCDO INOE 2000 Research Institute for Analytical Instrumentation Cluj Napoca Romania
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