Fate of mesoangioblasts in a vaginal birth injury model: influence of the route of administration
Language English Country Great Britain, England Media electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
30006567
PubMed Central
PMC6045600
DOI
10.1038/s41598-018-28967-w
PII: 10.1038/s41598-018-28967-w
Knihovny.cz E-resources
- MeSH
- Microscopy, Fluorescence MeSH
- Genetic Vectors chemistry genetics MeSH
- Wound Healing * MeSH
- Injections, Intralesional MeSH
- Injections, Intra-Arterial MeSH
- Injections, Intravenous MeSH
- Intravital Microscopy MeSH
- Rats MeSH
- Cells, Cultured MeSH
- Luciferases, Firefly chemistry genetics MeSH
- Rats, Sprague-Dawley MeSH
- Primary Cell Culture MeSH
- Pregnancy MeSH
- Mesenchymal Stem Cell Transplantation methods MeSH
- Vagina injuries MeSH
- Delivery, Obstetric adverse effects MeSH
- Green Fluorescent Proteins chemistry genetics MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Pregnancy MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Luciferases, Firefly MeSH
- Green Fluorescent Proteins MeSH
Currently cell therapy is considered as an experimental strategy to assist the healing process following simulated vaginal birth injury in rats, boosting the functional and morphologic recovery of pelvic floor muscles and nerves. However, the optimal administration route and dose still need to be determined. Mesangioblasts theoretically have the advantage that they can differentiate in skeletal and smooth muscle. We investigated the fate of mesoangioblasts transduced with luciferase and green fluorescent protein reporter genes (rMABseGFP/fLUC) using bioluminescence, immunofluorescence and RT-PCR in rats undergoing simulated birth injury. rMABseGFP/fLUC were injected locally, intravenously and intra-arterially (common iliacs and aorta). Intra-arterial delivery resulted in the highest amount of rMABseGFP/fLUC in the pelvic organs region and in a more homogeneous distribution over all relevant pelvic organs. Sham controls showed that the presence of the injury is important for recruitment of intra-arterially injected rMABseGFP/fLUC. Injection through the aorta or bilaterally in the common iliac arteries resulted in comparable numbers of rMABseGFP/fLUC in the pelvic organs, yet aortic injection was faster and gave less complications.
Centre for Surgical Technologies Group Biomedical Sciences KU Leuven Leuven Belgium
Department of Urology University Hospitals Leuven Leuven Belgium
Laboratory for Molecular Virology and Gene Therapy KU Leuven Flanders Belgium
Molecular Small Animal Imaging Center KU Leuven 3000 Leuven Belgium
Pelvic Floor Unit University Hospitals KU Leuven Leuven Belgium
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