Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry
Language English Country Germany Media print-electronic
Document type Journal Article
Grant support
CEITEC 2020 (LQ1601)
Ministry of Education, Youth and Sports of the Czech Republic
GACR 17-12774S
Grantová Agentura České Republiky
6SA17676
European Union's Horizon 2020 research
PubMed
30109381
DOI
10.1007/s00216-018-1300-7
PII: 10.1007/s00216-018-1300-7
Knihovny.cz E-resources
- Keywords
- Antibody labelling, Dot blot, Gold nanoparticles, Immunochemistry, LA-ICP-MS,
- MeSH
- Mass Spectrometry methods MeSH
- Immunoassay methods MeSH
- Indicators and Reagents chemistry MeSH
- Metal Nanoparticles chemistry MeSH
- Lasers * MeSH
- Limit of Detection MeSH
- Proof of Concept Study MeSH
- Antibodies chemistry MeSH
- Antibody Specificity MeSH
- Gold chemistry MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Indicators and Reagents MeSH
- Antibodies MeSH
- Gold MeSH
In this paper, we describe the labelling of antibodies by gold nanoparticles (AuNPs) with diameters of 10 and 60 nm with detection by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Additionally, the AuNPs labelling strategy is compared with commercially available labelling reagents based on MeCAT (metal coded affinity tagging). Proof of principle experiments based on dot blot experiments were performed. The two labelling methods investigated were compared by sensitivity and limit of detection (LOD). The absolute LODs achieved were in the range of tens of picograms for AuNP labelling compared to a few hundred picograms by the MeCAT labelling.
Bundesanstalt für Materialforschung und prüfung Richard Willstätter Straße 11 12489 Berlin Germany
CEITEC Masaryk University Kamenice 753 5 625 00 Brno Czech Republic
References provided by Crossref.org
Digital Immunoassay for Biomarker Detection Based on Single-Particle Laser Ablation ICP MS
Comparison of Metal Nanoparticles (Au, Ag, Eu, Cd) Used for Immunoanalysis Using LA-ICP-MS Detection
