Generation of human iPSCs from fetal prostate fibroblasts HPrF
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
30776675
DOI
10.1016/j.scr.2019.101405
PII: S1873-5061(19)30035-2
Knihovny.cz E-resources
- MeSH
- Fibroblasts * cytology metabolism MeSH
- Induced Pluripotent Stem Cells * cytology metabolism MeSH
- Kruppel-Like Factor 4 MeSH
- Humans MeSH
- Fetus * cytology embryology MeSH
- Cellular Reprogramming MeSH
- Prostate * cytology embryology MeSH
- Cellular Reprogramming Techniques * MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Human induced pluripotent stem cell line was generated from commercially available primary human prostate fibroblasts HPrF derived from a fetus, aged 18-24 weeks of gestation. The fibroblast cell line was reprogrammed with Yamanaka factors (OCT4, SOX2, c-MYC, KLF4) using CytoTune™-iPS 2.0 Sendai Reprogramming Kit. Pluripotency of the derived transgene-free iPS cell line was confirmed both in vitro by detecting the expression of factors of pluripotency on a single-cell level, and in vivo using teratoma formation assay. This iPS cell line will be a useful tool for studying both normal prostate development and prostate cancer disease.
Department of Histology and Embryology Faculty of Medicine Masaryk University Brno Czech Republic
Reprofit International Clinic of Reproductive Medicine Brno Czech Republic
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