Bacillus circulans 528 produces a restriction endonuclease, Bci528I which is an isoschizomer of EcoRI. We purified the enzyme, using Sephadex G-150, Phospho-cellulose, DEAE-cellulose, Hepharin-Sepharose CL-6B chromatography. The specific activity of Bci528I was 29,400 U/mg·protein. Bci528I recognizes 5'-GAATTC-3' in dsDNA and cleaves between G and A of the recognition sequence, producing a symmetric four base 5'overhang.

Korean Center for Culture Collection Pyongyang Democratic People's Republic of Korea

Research Institute of Biotechnology Faculty of Life Science Kim Il Sung University Pyongyang Democratic People's Republic of Korea

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