Synthesis of Base-Modified dNTPs Through Cross-Coupling Reactions and Their Polymerase Incorporation to DNA
Methods in molecular biology (Clifton, N.J.).
2019 ;
1973 () :
39-57.
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
Persistent link
https://www.medvik.cz/link/pmid31016695
- Keywords
- DNA polymerases, Enzymatic synthesis, Modified DNA, Nucleoside triphosphates, Nucleotides,
- MeSH
- DNA-Directed DNA Polymerase metabolism MeSH
- DNA biosynthesis chemistry MeSH
- Halogenation MeSH
- Nucleotides biosynthesis chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA-Directed DNA Polymerase MeSH
- DNA MeSH
- Nucleotides MeSH
Synthesis of base-modified dNTPs through the Suzuki or Sonogashira cross-coupling reactions of halogenated dNTPs with boronic acids or alkynes is reported, as well as the use of these modified dNTPs in polymerase incorporations to oligonucleotides or DNA by primer extension or PCR.
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