The method of long-term catheterization of the vena jugularis in pigs
Language English Country United States Media print-electronic
Document type Journal Article
PubMed
31100450
DOI
10.1016/j.vascn.2019.106584
PII: S1056-8719(19)30064-4
Knihovny.cz E-resources
- Keywords
- Jugular vein, Permanent intravenous catheterization, Pig,
- MeSH
- Catheterization methods MeSH
- Blood Specimen Collection methods MeSH
- Swine MeSH
- Taurine administration & dosage analogs & derivatives MeSH
- Thiadiazines administration & dosage MeSH
- Jugular Veins surgery MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Taurine MeSH
- taurolidine MeSH Browser
- Thiadiazines MeSH
INTRODUCTION: The pig is one of the most valuable in vivo models in biomedical research, however with only a few well-accessible veins suitable for venipuncture. Moreover, most of the known methods of blood collection are suitable only for a limited time period. The aim of the study was to verify an improved method of long-term catheterization of the jugular vein in pigs. METHODS: A 420 mm polyurethane catheter 16G tube was surgically inserted using the Seldinger technique. The part of the tube that was not inserted into the vein was threaded through a subcutaneously introduced trocar into the occipital area, where it was well accessible and well protected from damage. The catheters were flushed with sterile 0.9% saline solution and locked with 4% citrate between frequent blood samplings, or with 30% citrate at intervals of 1-2-days. Once a week, the catheters were locked with 4% citrate containing taurolidine for 24 h in order to prevent infection. The method was verified in 14 pigs. RESULTS: The catheters were fully functional for up to 11 weeks and no infection or thrombus was observed. DISCUSSION: This method of catheterization and catheter care allows the realization of long-term experiments with comfortable and stress-free blood sampling.
References provided by Crossref.org
