Brassinosteroids (BRs) are steroid hormones regulating various aspects of plant metabolism, including growth, development and stress responses. However, little is known about the mechanism of their impact on antioxidant systems and phospholipid turnover. Using tobacco plants overexpressing H+/Ca2+vacuolar Arabidopsis antiporter CAX1, we showed the role of Ca2+ ion balance in the reactive oxygen species production and rapid phosphatidic acid accumulation induced by exogenous BR. Combination of our experimental results with public transcriptomic and proteomic data revealed a particular role of Ca2+-dependent phospholipid metabolizing enzymes in BR signaling. Here we provide novel insights into the role of calcium balance and lipid-derived second messengers in plant responses to exogenous BRs and propose a complex model integrating BR-mediated metabolic changes with phospholipid turnover.

Department of the Molecular Mechanisms of Cell Metabolism Regulation 5 P Kukhar Institute of Bioorganic Chemistry and Petrochemistry The National Academy of Sciences of Ukraine 02660 Murmanska str 1 Kyiv Ukraine

Department of the Molecular Mechanisms of Cell Metabolism Regulation 5 P Kukhar Institute of Bioorganic Chemistry and Petrochemistry The National Academy of Sciences of Ukraine 02660 Murmanska str 1 Kyiv Ukraine; Laboratory of Pathological Plant Physiology Institute of Experimental Botany AS CR Rozvojová 263 165 02 Prague 6 Lysolaje Czech Republic

Institute of Food Biotechnology and Genomics National Academy of Sciences of Ukraine 04123 Osypovskogo 2a Kyiv Ukraine

Laboratory of Steroid Chemistry Institute of Bioorganic Chemistry The National Academy of Sciences of Belarus 220141 Kuprevich str 5 Minsk Belarus

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