In vitro testing of flash-frozen sublingual membranes for storage and reproducible permeability studies of macromolecular drugs from solution or nanofiber mats
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
31593804
DOI
10.1016/j.ijpharm.2019.118711
PII: S0378-5173(19)30756-2
Knihovny.cz E-resources
- Keywords
- Bovine serum albumin, Caffeine, Dextran, In vitro permeability, Nanofiber drug carrier, Porcine sublingual membrane, Sublingual drug administration,
- MeSH
- Albumins administration & dosage MeSH
- Administration, Sublingual MeSH
- Dextrans administration & dosage MeSH
- Fluorescein-5-isothiocyanate administration & dosage analogs & derivatives MeSH
- Caffeine administration & dosage MeSH
- Drug Delivery Systems * MeSH
- Nanofibers administration & dosage MeSH
- Permeability MeSH
- Swine MeSH
- Solutions MeSH
- Mucous Membrane anatomy & histology metabolism MeSH
- Freezing MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Albumins MeSH
- Dextrans MeSH
- fluorescein isothiocyanate dextran MeSH Browser
- Fluorescein-5-isothiocyanate MeSH
- Caffeine MeSH
- Solutions MeSH
Sublingual drug delivery allows systemic delivery of drug without difficulties connected with the gastrointestinal pathway. We developed a new simple protocol for easy-to-use processing and storage of porcine sublingual mucosal membrane for in vitro studies using "flash freezing" in liquid nitrogen. All the dextrans used as mucosal membrane integrity and permeability markers permeated only slowly through sublingual mucosa illustrating usability both the "fresh" and "flash frozen" sublingual membranes whereas conventional cold storage "frozen" membranes have shown significantly higher permeabilities for macromolecules due to the sustained damage. The permeability values were too low to expect dextrans to be potential carriers at this context. To test albumin as a drug carrier we compared FITC-albumin permeation from solutions vs. nanofiber mats donors. To increase the amounts and prolong the transport, we manufactured nanofiber mats loaded with fluorescently marked albumin using well-scalable electrospinning technology. Nanofiber mats have allowed albumin passage through the sublingual membrane in similar amounts as from the pure artificial saliva solution. Since salivary washout strictly limits the duration of liquid dosages, nanofiber mats may thus permit prolonged sublingual administration.
Department of Physical Chemistry RCPTM Faculty of Science Palacky University Olomouc Czech Republic
InStar Technologies a s Mrštíkova 399 2a 460 07 Liberec Czech Republic
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