Epoxidation is the preferred pathway of first-stage metabolism of abiraterone acetate in brown bullhead (Ameiurus nebulosus)
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články
Grantová podpora
NPU I Lo1509
ministry of education of the Czech Republic
LM2015051
ministry of education of the Czech Republic
CZ.02.1.01/0.0/16013/0001761
ministry of education of the Czech Republic
CZ.2.16/3.1.00/24023
Prague city hall
PubMed
31656995
DOI
10.1007/s11356-019-06568-y
PII: 10.1007/s11356-019-06568-y
Knihovny.cz E-zdroje
- Klíčová slova
- Abiraterone acetate, Ameiurus nebulosus, Epoxidation, Fish, HPLC/MS, Metabolites, NMR,
- MeSH
- abirateron metabolismus MeSH
- aromatické uhlovodíky metabolismus MeSH
- hmotnostní spektrometrie MeSH
- Ictaluridae fyziologie MeSH
- protinádorové látky metabolismus MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- abirateron MeSH
- aromatické uhlovodíky MeSH
- protinádorové látky MeSH
Twenty juvenile individuals of brown bullhead (Ameiurus nebulosus), average weight 77 g, were fed by abiraterone acetate prodrug dissolved in olive oil via gastric probe. Dose applied was 3 mg/10 g fish weight. After feeding, they were let out into aquarium and kept there for 3 days. Aquarium water containing excreted metabolites was extracted, and sample was purified and finally analyzed by means of HPLC/MS. Expected both primary (products of hydroxylation) and secondary (products of glucuronidation and sulfatation) metabolites of abiraterone acetate were identified. The NMR measurement of one of the prevailing metabolites presumed to be one of possible hydroxy-abiraterones discovered that it is not hydroxy-abiraterone but abiraterone 16,17-epoxide. Closer analysis of MS2 and MS3 spectra revealed that one of presumed hydroxy-abiraterone acetates and also some secondary metabolites are probably 16,17-epoxides.
Masaryk University Brno RECETOX Kamenice 126 3 625 00 Brno Czech Republic
Prague Institute of Chemical Technology Technická 5 166 28 Prague 6 Czech Republic
Teva Czech Industries s r o Ostravská 29 747 70 Opava Komárov Czech Republic
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