Capillary electrophoretic analysis of ionic content in exhaled breath condensate and pH monitoring as a non-invasive method in gastroesophageal reflux disease diagnostics
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
31790918
DOI
10.1016/j.jchromb.2019.121857
PII: S1570-0232(19)31329-7
Knihovny.cz E-resources
- Keywords
- Anions and cations, Capillary electrophoresis, Contactless conductivity detection, Exhaled breath condensate, Gastroesophageal reflux disease, pH,
- MeSH
- Butyrates analysis MeSH
- Breath Tests methods MeSH
- Adult MeSH
- Electrophoresis, Capillary methods MeSH
- Gastroesophageal Reflux diagnosis metabolism MeSH
- Ions analysis metabolism MeSH
- Hydrogen-Ion Concentration MeSH
- Middle Aged MeSH
- Humans MeSH
- Propionates analysis MeSH
- Aged MeSH
- Sodium analysis MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Butyrates MeSH
- Ions MeSH
- Propionates MeSH
- Sodium MeSH
In this study, the ionic profile and pH of exhaled breath condensate (EBC) in a group of patients with acid and weakly acid reflux and no-reflux controls were compared. A portable sampler was used for non-invasive EBC collection from five exhalations. The ionic profile (anions, cations, organic acids) and pH of the collected EBC samples were measured by capillary electrophoresis with contactless conductivity detection and a pH microelectrode, respectively. Several ions were elevated in the patient groups. Sodium cation was elevated in weakly acid reflux (significance level p < 0.01) and acid reflux (p < 0.05) compared to no-reflux controls. Butyrate and propionate were elevated in both acid reflux and weakly acid reflux compared to no-reflux controls (butyrate: p < 0.01, propionate: p < 0.05). The median values of pH (after de-aeration with N2) were also significantly higher (p < 0.01) in groups with acid reflux and weakly acid reflux than in the control group with no reflux. The ionic analysis and simultaneous pH measurement offer a simple, cheap, fast, and non-invasive approach in gastroesophageal reflux disease diagnostics.
References provided by Crossref.org
