Assessment of NK cell-mediated cytotoxicity by flow cytometry after rapid, high-yield isolation from peripheral blood
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
31948552
DOI
10.1016/bs.mie.2019.05.034
PII: S0076-6879(19)30204-6
Knihovny.cz E-resources
- Keywords
- Cytotoxicity assay, Degranulation assay, Flow cytometry, NK-cell mediated cytotoxicity,
- MeSH
- Lymphocyte Activation MeSH
- Killer Cells, Natural immunology MeSH
- Cytotoxicity, Immunologic * MeSH
- Cytotoxicity Tests, Immunologic methods MeSH
- Humans MeSH
- Flow Cytometry methods MeSH
- Cell Separation methods MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Natural killer (NK) cells constitute the predominant innate lymphocyte subset that mediates the anti-viral and anti-tumor immune responses. NK cells use an array of innate receptors to sense their environment and to respond to infections, cellular stress and transformation. The resulting NK cell activation, including cytotoxicity and cytokine production, is a fundamental component of the early immune response. The most recent discoveries in NK cell biology have stimulated the translational research that has led to remarkable results for the treatment of human malignancies. Therefore, the rapid isolation of NK cells from the peripheral blood or tumor microenvironment and the subsequent assessment of cytolytic function are crucial to the study of their potency and NK cell-mediated immunosurveillance. Here, we provide protocols for NK cell isolation and the assessment of NK cell cytotoxicity using flow cytometry.
Biomedical Center Medical Faculty in Pilsen Charles University Pilsen Czech Republic
Department of Hematology and Oncology Hospital in Pilsen Pilsen Czech Republic
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