Exposure to Spectracide® causes behavioral deficits in Drosophila melanogaster: Insights from locomotor analysis and molecular modeling
Language English Country Great Britain, England Media print-electronic
Document type Journal Article
PubMed
32018111
DOI
10.1016/j.chemosphere.2020.126037
PII: S0045-6535(20)30230-7
Knihovny.cz E-resources
- Keywords
- Behavioral toxicology, Carbonyl reductase, Drosophila melanogaster, Ecotoxicology, In silico modeling, Spectracide®,
- MeSH
- Alcohol Oxidoreductases metabolism MeSH
- Atrazine toxicity MeSH
- Behavior, Animal drug effects MeSH
- Drosophila melanogaster genetics MeSH
- Gene Expression MeSH
- Herbicides toxicity MeSH
- Locomotion drug effects MeSH
- Models, Molecular * MeSH
- Oxidation-Reduction MeSH
- Oxidative Stress genetics MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- alcohol dehydrogenase (NADP+) MeSH Browser
- Alcohol Oxidoreductases MeSH
- Atrazine MeSH
- Herbicides MeSH
This study was focused on gaining insights into the mechanism by which the herbicide- Spectracide®, induces oxidative stress and alters behavior in Drosophila melanogaster. Exposure to Spectracide® (50%) significantly (p < 0.05) reduced the negative geotaxis response, jumping behavior and dampened locomotor activity rhythm in adult flies compared to non-exposed flies. Protein carbonyl levels indicative of oxidative damage increased significantly coupled with down-regulation of Sniffer gene expression encoding carbonyl reductase (CR) and its activity in Spectracide®-exposed flies. In silico modeling analysis revealed that the active ingredients of Spectracide® (atrazine, diquat dibromide, fluazifop-p-butyl, and dicamba) have significant binding affinity to the active site of CR enzyme, with atrazine having comparatively greater affinity. Our results suggest a mechanism by which ingredients in Spectracide® induce oxidative damage by competitive binding to the active site of a protective enzyme and impair its ability to prevent damage to proteins thereby leading to deficits in locomotor behavior in Drosophila.
Biology Department Stillman College Tuscaloosa AL 35404 USA
Department of Biological Sciences University of Alabama Tuscaloosa AL 35487 USA
Department of Microbiology West Bengal State University Barasat Kolkata 126 India
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